Learning with NCCAT Staff

Join us for regularly scheduled remote sessions with our staff.


NCCAT Roundtables, are virtual gatherings of small peer-groups, providing an open forum to discuss both specific or general questions on a focused topic in cryoEM. We start with a 5-10 minute intro given by our staff and then open up the floor for Q&A/discussion. The schedule of topics for our past series is below. New series will be scheduled based on demand. If you have a particular interest in a topic not listed, contact us at nccattraining [at] nysbc.org and we will hold additional sessions for topics in high demand. Roundtables last ~1 hour.

Future Dates: Registration is open to all, you can register for multiple dates but will have to register for each date individually. Links will be provided here when dates and topics are scheduled.

April 9: Data processing to 2D averages

April 16: Processing in the cloud (AWS tutorial)

April 23: Tomography part 1 – theory

April 30: Tomography part 2 – practice

May 7: SPA Membrane protein data processing

May 14: Practical strategies for efficient Krios data collection

May 21: Data processing – generating 3D reconstructions

May 28: Grid preparation for single particle analysis

June 4: Blot-free vitrification and nanowire grids

April 20: Sample prep: What grids should I use?

May 4: Data collection: How much will I benefit from the new TEM hardware?

May 18: Data collection: What is “screening”? How do I use on-the-fly feedback?

June 1: Data processing: SPA – 3D refinement, symmetry expansion and masking

June 15: CryoEM facility logistics: Tracking and management for different user bases

June 29: SPA workflows from Data to map, journal club style: Evaluate the methods & data from a paper of interest (paper TBD)

July 13, 9:00 AM: Machine learning: What steps in the pipeline are benefiting and how do I trust it? [Note different meeting time!]

July 27: SPA Tricks for tricky samples (membrane proteins, preferred orientations, etc…)

August 10: Sample prep for electron diffraction with 3D-crystals

August 24: SPA model building & validation

September 7: MicroED: Data collection strategies

 September 21: Data processing:  Tomography – sub-tomo averaging workflows

Office hours

Tuesdays at 3:00 PM Eastern Time we hold one-on-one office hours for trainees to address topics including:

  • Questions you have after using on our online course materials
  • Specific questions about your project, from sample prep to data processing and analysis. If you’ve collected data at NCCAT we can dive into your data together
  • How to access and best use our instrumentation resources

To schedule an office hours appointment, contact us at nccatinfo [at] nysbc.org.