• 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • March 14-18, 2022.

  • At no cost.

  • Room and board NOT included.

Application deadline passed

To join the waitlist or for more information email nccatuseroffice [at] nysbc.org

see the 2020 shortcourse

Single Particle Short Course
March 14-18 2022

NCCAT offered a 1 week workshop focused on the theory and practice of single-particle analysis. The mornings were filled with lectures and stimulating round table discussions. The afternoons provided hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.

Short-course Agenda

Monday, March 14, 2022

Session Type Lecturer Topic
Morning Lecture 1 – Keynote Joachim Frank (Columbia University) Intro and overview of SPA [pdf][lecture video]
Morning Roundtable 1 students Flashtalks from students
Afternoon Lecture 2 Victor Chen & Wen Jian (Purdue University) cryoVR for sample preparation [pdf][lecture video]
Afternoon Practical 1 SEMC staff cryoEM sample prep and grid prep stations [stations]

Tuesday, March 15, 2022

Session Type Lecturer Topic
Morning Lecture 3 SEMC staff Microscopes and tools of the trade [pdf][lecture video]
Afternoon Roundtable 2 SEMC staff Making use of local facilities and national centers
Afternoon Practical 2 SEMC staff Plunge freezing & TEM stations [stations][grid prep note page]

Wednesday, March 16, 2022

Session Type Lecturer Topic
Morning Lecture 4a Fred Sigworth (Yale University) Algorithms and foundational math PartI:
derivation/explanation of the CTF, FTs. [pdf][lecture video]
Morning Lecture 4b Fred Sigworth (Yale University) Algorithms and foundational math PartII:
reconstruction, classification, maximum likelihood. [pdf][lecture video]
Morning Lecture 5 Amedee des Georges & Reza Khayat (ASRC/CUNY) Data Analysis and reconstruction workflow [pdf][lecture video]
Lunch Roundtable 3 Fred Sigworth & Amedee des Georges Current and new algorithms to deal with SPA reconstruction [lecture video]
Afternoon Practical 3 SEMC staff Processing using cryoSPARC [instructions]

Thursday, March 17, 2022

Session Type Lecturer Topic
Morning Lecture 6 Rich Hite (Memorial Sloan Kettering Cancer Center) Interpretation and Limitations of SPA [pdf][lecture video]
Morning Lecture 7 Tom Walz (Rockefeller University) Validation Methods [pdf][lecture video]
Lunch Roundtable 4 Rich Hite & Tom Walz EM challenges and new frontiers
Afternoon Practical 4 SEMC staff Autoloader TEMs and Krios data collection at a national center [stations]
Afternoon Lecture 8 Cathy Lawson (Rutgers University) EMDataResource: Structure Data Archiving, Validation Challenges [pdf][lecture video]
Dinner all instructors Group dinner

Friday, March 18, 2022

Session Type Lecturer Topic
Morning Lecture 9 Oli Clarke (Columbia University) Fitting Atomic Models [pdf][lecture video]
Morning Lecture 10 Gira Bhabha & Damian Ekiert (New York University) Coordinate Refinement and Validation [pdf][lecture video]
Morning Roundtable 5 Oli Clarke, Gira Bhabha and Damian Ekiert Making biological conclusions from cryoEM reconstructions.
Afternoon Practical 5 Oli Clarke Coot and model building [instructions]

This workshop is offered at NO COST, but limited to 16 attendees.

Join the waitlist.

We will reach out to participants by end of January to the beginning of February. For further inquires please email the NCCAT User Office.

Yes. This will be an in-person on-site workshop. All attendees should be prepared to provide a CDC vaccination card or show their New York State Excelsior Pass showing proof of COVID-19 vaccination.

A copy of the NYSBC’s COVID-19 policies may be found here.

Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.