Publications
2024
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Aragon M, Bowman SEJ, Chen CH, de la Cruz MJ, Decato DA, Eng ET, et al. Applying 3D ED/MicroED workflows toward the next frontiers. Acta Crystallogr C Struct Chem. 2024.
- Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sanchez R, et al. Structures, functions and adaptations of the human LINE-1 ORF2 protein. Nature. 2024.
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Begley M, Aragon M, Baker RW. A structure-based mechanism for initiation of AP-3 coated vesicle formation. bioRxiv. 2024.
- Bha Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, et al. Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19. Nature. 2024.
- Clark J, Hoxie I, Adelsberg DC, Sapse IA, Andreata-Santos R, Yong JS, et al. Protective effect and molecular mechanisms of human non-neutralizing cross-reactive spike antibodies elicited by SARS-CoV-2 mRNA vaccination. bioRxiv. 2024.
- Decato D, Palatinus L, Stierle A, Stierle D. Absolute structure determination of Berkecoumarin by X-ray and electron diffraction. Acta Crystallogr C Struct Chem. 2024.
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Ennist NM, Wang S, Kennedy MA, Curti M, Sutherland GA, Vasilev C, et al. De novo design of proteins housing excitonically coupled chlorophyll special pairs. Nat Chem Biol. 2024.
- Feng S, Aplin C, Nguyen TT, Milano SK, Cerione RA. Filament formation drives catalysis by glutaminase enzymes important in cancer progression. Nat Commun. 2024.
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Gangwar SP, Yelshanskaya MV, Nadezhdin KD, Yen LY, Newton TP, Aktolun M, et al. Kainate receptor channel opening and gating mechanism. Nature. 2024.
- Hou K, Ge P, Sawaya MR, Dolinsky JL, Yang Y, Jiang YX, et al. How short peptides can disassemble ultra-stable tau fibrils extracted from Alzheimer’s disease brain by a strain-relief mechanism. bioRxiv. 2024.
- Huddy TF, Hsia Y, Kibler RD, Xu J, Bethel N, Nagarajan D, et al. Blueprinting extendable nanomaterials with standardized protein blocks. Nature. 2024.
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Jaiswal R, Santosh V, Braud B, Washington A, Escalante CR. Cryo-EM Structure of AAV2 Rep68 bound to integration site AAVS1: Insights into the mechanism of DNA melting. bioRxiv. 2024.
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Jia L, Ruben EA, Suarez HJ, Olsen SK, Wasmuth EV. Single particle cryo-electron microscopy with an enhanced 200 kV cryo-TEM configuration achieves near-atomic resolution. bioRxiv. 2024.
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Li J, Li Y, Koide A, Kuang H, Torres VJ, Koide S, et al. Proton-coupled transport mechanism of the efflux pump NorA. Nat Commun. 2024.
- Nguyen BA, Singh V, Afrin S, Yakubovska A, Wang L, Ahmed Y, et al. Structural polymorphism of amyloid fibrils in ATTR amyloidosis revealed by cryo-electron microscopy. Nat Commun. 2024.
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Olia AS, Prabhakaran M, Harris DR, Cheung CS, Gillespie RA, Gorman J, et al. Anti-idiotype isolation of a broad and potent influenza A virus-neutralizing human antibody. Front Immunol. 2024.
- Sharma S, Luo M, Patel H, Mueller DM, Liao M. Conformational ensemble of yeast ATP synthase at low pH reveals unique intermediates and plasticity in F(1)-F(o) coupling. Nat Struct Mol Biol. 2024.
- Wang L, Hoang A, Gil-Iturbe E, Laganowsky A, Quick M, Zhou M. Mechanism of anion exchange and small-molecule inhibition of pendrin. Nat Commun. 2024.
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Warren AL, Lankri D, Cunningham MJ, Serrano IC, Parise LF, Kruegel AC, et al. Structural pharmacology and therapeutic potential of 5-methoxytryptamines. Nature. 2024.
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Xu D, Ando N. Miffi: Improving the accuracy of CNN-based cryo-EM micrograph filtering with fine-tuning and Fourier space information. J Struct Biol. 2024.
- Yang R, Ko YH, Li F, Lokareddy RK, Hou CD, Kim C, et al. Structural basis for nuclear import of hepatitis B virus (HBV) nucleocapsid core. Sci Adv. 2024.
- Zilberg G, Parpounas AK, Warren AL, Yang S, Wacker D. Molecular basis of human trace amine-associated receptor 1 activation. Nat Commun. 2024.
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Zilberg G, Parpounas AK, Warren AL, Fiorillo B, Provasi D, Filizola M, et al. Structural insights into the unexpected agonism of tetracyclic antidepressants at serotonin receptors 5-HT(1e)R and 5-HT(1F)R. Sci Adv. 2024.
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Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, et al. Promiscuous G-protein activation by the calcium-sensing receptor. Nature. 2024.
2023
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Atanasoff KE, Brambilla L, Adelsberg DC, Kowdle S, Stevens CS, Hung CT, et al. An in vitro experimental pipeline to characterize the binding specificity of SARS-CoV-2 neutralizing antibodies. bioRxiv. 2023.
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Capper MJ, Yang S, Stone AC, Vatansever S, Zilberg G, Mathiharan YK, et al. Substrate binding and inhibition of the anion exchanger 1 transporter. Nat Struct Mol Biol. 2023.
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Consalvo CD, Aderounmu AM, Donelick HM, Aruscavage PJ, Eckert DM, Shen PS, et al. C. elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA. bioRxiv. 2023.
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Edman NI, Redler RL, Phal A, Schlichthaerle T, Srivatsan SR, Etemadi A, et al. Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies. bioRxiv. 2023.
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Feng S, Aplin C, Nguyen TT, Cerione RA. Filament formation by glutaminase enzymes drives catalysis. bioRxiv. 2023.
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Frey SJ, Carreno JM, Bielak D, Arsiwala A, Altomare CG, Varner C, et al. Nanovaccines Displaying the Influenza Virus Hemagglutinin in an Inverted Orientation Elicit an Enhanced Stalk-Directed Antibody Response. Adv Healthc Mater. 2023.
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Gangwar SP, Yen LY, Yelshanskaya MV, Sobolevsky AI. Positive and negative allosteric modulation of GluK2 kainate receptors by BPAM344 and antiepileptic perampanel. Cell reports. 2023.
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Gibbs E, Klemm E, Seiferth D, Kumar A, Ilca SL, Biggin PC, et al. Conformational transitions and allosteric modulation in a heteromeric glycine receptor. Nat Commun. 2023.
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Han BG, Avila-Sakar A, Remis J, Glaeser RM. Challenges in making ideal cryo-EM samples. Curr Opin Struct Biol. 2023.
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Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, et al. Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage. Nat Commun. 2023.
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Hoang Trinh TK, Catalano C, Guo Y. Fabrication of membrane proteins in the form of native cell membrane nanoparticles using novel membrane active polymers. Nanoscale Adv. 2023.
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Hu Z, Zheng X, Yang J. Conformational trajectory of allosteric gating of the human cone photoreceptor cyclic nucleotide-gated channel. Nat Commun. 2023.
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Huddy TF, Hsia Y, Kibler RD, Xu J, Bethel N, Nagarajan D, et al. Blueprinting expandable nanomaterials with standardized protein building blocks. bioRxiv. 2023.
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Kulczyk AW, Sorzano COS, Grela P, Tchorzewski M, Tumer NE, Li XP. Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk reveals residues involved in the binding interaction. J Biol Chem. 2023.
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Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, et al. Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nat Commun. 2023.
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Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, et al. High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. Nat Commun. 2023.
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Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, et al. Structural basis for substrate selection by the SARS-CoV-2 replicase. Nature. 2023.
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Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH. Structural basis of Rho-dependent transcription termination. Nature. 2023.
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Monsen RC, Chua EYD, Hopkins JB, Chaires JB, Trent JO. Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 A resolution with cryo-EM. Nucleic Acids Res. 2023.
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Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, et al. A general mechanism for transcription bubble nucleation in bacteria. Proc Natl Acad Sci U S A. 2023.
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Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, et al. Structures of the holo CRISPR RNA-guided transposon integration complex. Nature. 2023.
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Patel A, Kumar S, Lai L, Chakravarthy C, Valanparambil R, Reddy ES, et al. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response. Structure. 2023.
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Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, et al. Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays. Nat Struct Mol Biol. 2023.
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Wang C, Molodtsov V, Sanders TJ, Marshall CJ, Firlar E, Kaelber JT, et al. Structural basis of archaeal FttA-dependent transcription termination. bioRxiv. 2023.
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Watkins MB, Wang H, Burnim A, Ando N. Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryo-electron microscopy. bioRxiv. 2023.
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Weaver JW, Proshkin S, Duan W, Epshtein V, Gowder M, Bharati BK, et al. Control of transcription elongation and DNA repair by alarmone ppGpp. Nat Struct Mol Biol. 2023.
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Wilbon AS, Shen J, Ruchala P, Zhou M, Pan Y. Structural basis of ferroportin inhibition by minihepcidin PR73. PLoS Biol. 2023.
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Yang Y, Fritzsching KJ, He S, McDermott AE. Zinc Alters the Supramolecular Organization of Nucleic Acid Complexes with Full-Length TIA1. bioRxiv. 2023.
2022
- Capper MJ, Yang S, Stone AC, Vatansever S, Zilberg G, Mathiharan YK, Habib R, Hutchinson K, Schlessinger A, Mezei M, Osman R, Zhang B, Wacker D. Substrate Binding and Inhibition of the Anion Exchanger 1 Transporter. bioRxiv. 2022. [preprint].
- Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA. Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex. Nat Struct Mol Biol. 2022;29(3):250-260.
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Finkelstein MT, Parker Miller E, Erdman MC, Fera D. Analysis of two cooperating antibodies unveils immune pressure imposed on HIV Env to elicit a V3-glycan supersite broadly neutralizing antibody lineage. Front Immunol. 2022.
- Gobeil SMC, Henderson R, Stalls V, Janowska K, Huang X, May A, Speakman M, Beaudoin E, Manne K, Li D, Parks R, Barr M, Deyton M, Martin M, Mansouri K, Edwards RJ, Sempowski GD, Saunders KO, Wiehe K, Williams W, Korber B, Haynes BF, Acharya P. Structural diversity of the SARS-CoV-2 Omicron spike. bioRxiv. 2022.
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Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, et al. Structural basis for breadth development in a HIV-1 neutralizing antibody. bioRxiv. 2022.
- Kendall AK, Chandra M, Xie B, Wan W, Jackson LP. Improved mammalian retromer cryo-EM structures reveal a new assembly interface. bioRxiv. 2022.
- Klykov O, Kopylov M, Carragher B, Heck AJR, Noble AJ, Scheltema RA. Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology. Mol Cell. 2022.
- Koo CW, Tucci FJ, He Y, Rosenzweig AC. Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer. Science. 2022.
- Laughlin ZT, Nandi S, Dey D, Zelinskaya N, Witek MA, Srinivas P, Nguyen HA, Kuiper EG, Comstock LR, Dunham CM, Conn GL. 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA. Proc Natl Acad Sci U S A. 2022.
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Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, et al. CryoEM Structures of the Human HIV-1 Restriction Factor SERINC3 and Function as a Lipid Transporter. bioRxiv. 2022.
- Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ. Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition. Nat Commun. 2022.
- Malone BF, Perry JK, Olinares PDB, Chen J, Appelby TK, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Chait BT, Campbell EA, Darst SA. Structural basis for substrate selection by the SARS-CoV-2 replicase. bioRxiv. 2022.
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Nguyen BA, Afrin S, Singh V, Ahmed Y, Pedretti R, del Carmen Fernandez-Ramirez M, et al. Structural polymorphism of amyloid fibrils in cardiac ATTR amyloidosis revealed by cryo-electron microscopy. bioRxiv. 2022.
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Patel A, Kumar S, Lai L, Chakravarthy C, Valanparambil R, Reddy ES, et al. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response. bioRxiv. 2022.
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Smith EM, Ferrell S, Tokars VL, Mondragon A. Structures of an active type III-A CRISPR effector complex. Structure. 2022.
- Trinh TKH, Catalano C, Guo Y. Membrane-active Polymers: NCMNP13-x, NCMNP21-x and NCMNP21b-x for Membrane Protein Structural Biology. bioRxiv. 2022.
- Tsai K, Stojkovic V, Lee DJ, Young ID, Szal T, Klepacki D, Vazquez-Laslop N, Mankin AS, Fraser JS, Fujimori DG. Structural basis for context-specific inhibition of translation by oxazolidinone antibiotics. Nat Struct Mol Biol. 2022.
- Wasmuth EV, Broeck AV, LaClair JR, Hoover EA, Lawrence KE, Paknejad N, Pappas K, Matthies D, Wang B, Feng W, Watson PA, Zinder JC, Karthaus WR, de la Cruz MJ, Hite RK, Manova-Todorova K, Yu Z, Weintraub ST, Klinge S, Sawyers CL. Allosteric interactions prime androgen receptor dimerization and activation. bioRxiv. 2022. [preprint].
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Wilbon AS, Shen J, Ruchala P, Zhou M, Pan Y. Structural basis of ferroportin inhibition by minihepcidin PR73. bioRxiv. 2022.
- Yelshanskaya MV, Patel DS, Kottke CM, Kurnikova MG, Sobolevsky AI. Opening of glutamate receptor channel to subconductance levels. Nature. 2022.
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Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, et al. Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP(2). Science. 2022.
- Yin Z, Bird JG, Kaelber JT, Nickels BE, Ebright RH. Structural basis of transcription antitermination by Qλ: NusA induces refolding of Qλ to form nozzle for RNA polymerase exit channel. bioRxiv. 2022. [preprint]
- Yu Y, Li S, Ser Z, Kuang H, Than T, Guan D, Zhao X, Patel DJ. Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes. Proc Natl Acad Sci U S A. 2022.
- Zhao Y, Roy K, Vidossich P, Cancedda L, De Vivo M, Forbush B, Cao E. Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide. Nat Commun. 2022;13(1):2747.
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Zhao Y, Shen J, Wang Q, Ruiz Munevar MJ, Vidossich P, De Vivo M, et al. Structure of the human cation-chloride cotransport KCC1 in an outward-open state. Proc Natl Acad Sci U S A. 2022.
- Zheng X, Hu Z, Li H, Yang J. Structure of the human cone photoreceptor cyclic nucleotide-gated channel. Nat Struct Mol Biol. 2022;29(1):40-46.
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Zhou W, Yadav GP, Yang X, Qin F, Li C, Jiang QX. Cryo-EM structure-based selection of computed ligand poses enables design of MTA-synergic PRMT5 inhibitors of better potency. Commun Biol. 2022.
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Zhu J, Huang W, Zhao J, Huynh L, Taylor DJ, Harris ME. Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P. Nat Commun. 2022.
- Zimanyi CM, Kopylov M, Potter CS, Carragher B, Eng ET. Broadening access to cryoEM through centralized facilities. Trends Biochem Sci. 2022.
2021
- Catalano C, Ben-Hail D, Qiu W, Blount P, des Georges A, Guo Y. Cryo-EM Structure of Mechanosensitive Channel YnaI Using SMA2000: Challenges and Opportunities. Membranes. 2021;11(11):849.
- Cerutti G, Guo Y, Zhou T, Gorman J, Lee M, Rapp M, Reddem ER, Yu J, Bahna F, Bimela J, Huang Y, Katsamba PS, Liu L, Nair MS, Rawi R, Olia AS, Wang P, Zhang B, Chuang GY, Ho DD, Sheng Z, Kwong PD, Shapiro L. Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite. Cell Host Microbe. 2021:2021.2001.2010.426120.
- Cerutti G, Rapp M, Guo Y, Bahna F, Bimela J, Reddem ER, Yu J, Wang P, Liu L, Huang Y, Ho DD, Kwong PD, Sheng Z, Shapiro L. Structural basis for accommodation of emerging B.1.351 and B.1.1.7 variants by two potent SARS-CoV-2 neutralizing antibodies. Structure. 2021;29(7):655-663 e654.
- Chen K-E, Guo Q, Cui Y, Kendall AK, Hill TA, Hall RJ, Sacharz J, Norwood SJ, Xie B, Leneva N, Yang Z, Ghai R, Stroud DA, Fairlie D, Suga H, Jackson LP, Teasdale RD, Passioura T, Collins BM. De novo macrocyclic peptides for inhibiting, stabilising and probing the function of the Retromer endosomal trafficking complex. bioRxiv. 2021. [preprint]
- Feldman J, Bals J, Altomare CG, St Denis K, Lam EC, Hauser BM, Ronsard L, Sangesland M, Bracamonte Moreno T, Okonkwo V, Hartojo N, Balazs AB, Bajic G, Lingwood D, Schmidt AG. Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses. Sci Immunol. 2021:eabl5842.
- Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Cell reports. 2021;34(2):108630.
- Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Stalls V, Kopp MF, Manne K, Li D, Wiehe K, Saunders KO, Edwards RJ, Korber B, Haynes BF, Henderson R, Acharya P. Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. Science. 2021;373(6555).
- He Y, Wang Y, Liu B, Helmling C, Sušac L, Cheng R, Zhou ZH, Feigon J. Structures of telomerase at several steps of telomere repeat synthesis. Nature. 2021;593(7859):454-459.
- Herrera NG, Morano NC, Celikgil A, Georgiev GI, Malonis RJ, Lee JH, Tong K, Vergnolle O, Massimi AB, Yen LY, Noble AJ, Kopylov M, Bonanno JB, Garrett-Thomson SC, Hayes DB, Bortz RH, 3rd, Wirchnianski AS, Florez C, Laudermilch E, Haslwanter D, Fels JM, Dieterle ME, Jangra RK, Barnhill J, Mengotto A, Kimmel D, Daily JP, Pirofski LA, Chandran K, Brenowitz M, Garforth SJ, Eng ET, Lai JR, Almo SC. Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis. ACS Omega. 2021;6(1):85-102.
- Klykov O, Gangwar SP, Yelshanskaya MV, Yen L, Sobolevsky AI. Structure and desensitization of AMPA receptor complexes with type II TARP gamma5 and GSG1L. Mol Cell. 2021.
- Kumar P, Cymes GD, Grosman C. Structure and function at the lipid-protein interface of a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A. 2021;118(23):e2100164118.
- Li D, Edwards RJ, Manne K, Martinez DR, Schafer A, Alam SM, Wiehe K, Lu X, Parks R, Sutherland LL, Oguin TH, 3rd, McDanal C, Perez LG, Mansouri K, Gobeil SMC, Janowska K, Stalls V, Kopp M, Cai F, Lee E, Foulger A, Hernandez GE, Sanzone A, Tilahun K, Jiang C, Tse LV, Bock KW, Minai M, Nagata BM, Cronin K, Gee-Lai V, Deyton M, Barr M, Von Holle T, Macintyre AN, Stover E, Feldman J, Hauser BM, Caradonna TM, Scobey TD, Rountree W, Wang Y, Moody MA, Cain DW, DeMarco CT, Denny TN, Woods CW, Petzold EW, Schmidt AG, Teng IT, Zhou T, Kwong PD, Mascola JR, Graham BS, Moore IN, Seder R, Andersen H, Lewis MG, Montefiori DC, Sempowski GD, Baric RS, Acharya P, Haynes BF, Saunders KO. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell. 2021.
- Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA. Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex. Proc Natl Acad Sci U S A. 2021;118(19).
- Moghadamchargari Z, Shirzadeh M, Liu C, Schrecke S, Packianathan C, Russell DH, Zhao M, Laganowsky A. Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants. Proc Natl Acad Sci U S A. 2021;118(12).
- Nadezhdin KD, Neuberger A, Nikolaev YA, Murphy LA, Gracheva EO, Bagriantsev SN, Sobolevsky AI. Extracellular cap domain is an essential component of the TRPV1 gating mechanism. Nat Commun. 2021;12(1):2154.
- Olinares PDB, Kang JY, Llewellyn E, Chiu C, Chen J, Malone B, Saecker RM, Campbell EA, Darst SA, Chait BT. Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM. Structure. 2021;29(2):186-195 e186.
- Nadezhdin KD, Neuberger A, Trofimov YA, Krylov NA, Sinica V, Kupko N, Vlachova V, Zakharian E, Efremov RG, Sobolevsky AI. Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel. Nat Struct Mol Biol. 2021;28(7):564-572.
- Rangarajan ES, Izard T. The Cryogenic Electron Microscopy Structure of the Cell Adhesion Regulator Metavinculin Reveals an Isoform-Specific Kinked Helix in Its Cytoskeleton Binding Domain. Int J Mol Sci. 2021;22(2).
- Rapp M, Guo Y, Reddem ER, Yu J, Liu L, Wang P, Cerutti G, Katsamba P, Bimela JS, Bahna FA, Mannepalli SM, Zhang B, Kwong PD, Huang Y, Ho DD, Shapiro L, Sheng Z. Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class. Cell reports. 2021;35(1):108950.
- Sauer DB, Wang B, Sudar JC, Song J, Marden J, Rice WJ, Wang DN. The ups and downs of elevator-type di-/tricarboxylate membrane transporters. FEBS J. 2021.
- Schmitz AJ, Turner JS, Liu Z, Zhou JQ, Aziati ID, Chen RE, Joshi A, Bricker TL, Darling TL, Adelsberg DC, Altomare CG, Alsoussi WB, Case JB, VanBlargan LA, Lei T, Thapa M, Amanat F, Jeevan T, Fabrizio T, O’Halloran JA, Shi PY, Presti RM, Webby RJ, Krammer F, Whelan SPJ, Bajic G, Diamond MS, Boon ACM, Ellebedy AH. A vaccine-induced public antibody protects against SARS-CoV-2 and emerging variants. Immunity. 2021;54(9):2159-2166 e2156.
- Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H. Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity. 2021.
- Sun D, Sang Z, Kim YJ, Xiang Y, Cohen T, Belford AK, Huet A, Conway JF, Sun J, Taylor DJ, Schneidman-Duhovny D, Zhang C, Huang W, Shi Y. Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopes. Nat Commun. 2021;12(1):4676.
- van Eeuwen T, Shim Y, Kim HJ, Zhao T, Basu S, Garcia BA, Kaplan CD, Min JH, Murakami K. Cryo-EM structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in nucleotide excision repair. Nat Commun. 2021;12(1):3338.
- Wang JC, Chen L. Structural basis for the structural dynamics of human mitochondrial chaperonin mHsp60. Sci Rep. 2021;11(1):14809.
- Williams WB, Meyerhoff RR, Edwards RJ, Li H, Manne K, Nicely NI, Henderson R, Zhou Y, Janowska K, Mansouri K, Gobeil S, Evangelous T, Hora B, Berry M, Abuahmad AY, Sprenz J, Deyton M, Stalls V, Kopp M, Hsu AL, Borgnia MJ, Stewart-Jones GBE, Lee MS, Bronkema N, Moody MA, Wiehe K, Bradley T, Alam SM, Parks RJ, Foulger A, Oguin T, Sempowski GD, Bonsignori M, LaBranche CC, Montefiori DC, Seaman M, Santra S, Perfect J, Francica JR, Lynn GM, Aussedat B, Walkowicz WE, Laga R, Kelsoe G, Saunders KO, Fera D, Kwong PD, Seder RA, Bartesaghi A, Shaw GM, Acharya P, Haynes BF. Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. Cell. 2021;184(11):2955-2972 e2925.
- Yang D, Gouaux E. Illumination of serotonin transporter mechanism and role of the allosteric site. Sci Adv. 2021;7(49):eabl3857.
2020
- Acharya P, Williams W, Henderson R, Janowska K, Manne K, Parks R, Deyton M, Sprenz J, Stalls V, Kopp M, Mansouri K, Edwards RJ, Meyerhoff RR, Oguin T, Sempowski G, Saunders K, Haynes BF. A glycan cluster on the SARS-CoV-2 spike ectodomain is recognized by Fab-dimerized glycan-reactive antibodies. bioRxiv. 2020. [preprint]
- Chen J, Malone B, Llewellyn E, Grasso M, Shelton PMM, Olinares PDB, Maruthi K, Eng ET, Vatandaslar H, Chait BT, Kapoor TM, Darst SA, Campbell EA. Structural Basis for Helicase-Polymerase Coupling in the SARS-CoV-2 Replication-Transcription Complex. Cell. 2020;182(6):1560-1573 e1513.
- Durie CL, Sheedlo MJ, Chung JM, Byrne BG, Su M, Knight T, Swanson M, Lacy DB, Ohi MD. Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex. Elife. 2020;9.
- Henderson R, Edwards RJ, Mansouri K, Janowska K, Stalls V, Gobeil SMC, Kopp M, Li D, Parks R, Hsu AL, Borgnia MJ, Haynes BF, Acharya P. Controlling the SARS-CoV-2 spike glycoprotein conformation. Nat Struct Mol Biol. 2020;27(10):925-933.
- Li Q, Pellegrino J, Lee DJ, Tran AA, Chaires HA, Wang R, Park JE, Ji K, Chow D, Zhang N, Brilot AF, Biel JT, van Zundert G, Borrelli K, Shinabarger D, Wolfe C, Murray B, Jacobson MP, Muhle E, Chesneau O, Fraser JS, Seiple IB. Synthetic group A streptogramin antibiotics that overcome Vat resistance. Nature. 2020;586(7827):145-150.
- Liu L, Wang P, Nair MS, Yu J, Rapp M, Wang Q, Luo Y, Chan JF, Sahi V, Figueroa A, Guo XV, Cerutti G, Bimela J, Gorman J, Zhou T, Chen Z, Yuen KY, Kwong PD, Sodroski JG, Yin MT, Sheng Z, Huang Y, Shapiro L, Ho DD. Potent neutralizing antibodies against multiple epitopes on SARS-CoV-2 spike. Nature. 2020;584(7821):450-456.
- Santosh V, Musayev FN, Jaiswal R, Zarate-Perez F, Vandewinkel B, Dierckx C, Endicott M, Sharifi K, Dryden K, Henckaerts E, Escalante CR. The Cryo-EM structure of AAV2 Rep68 in complex with ssDNA reveals a malleable AAA+ machine that can switch between oligomeric states. Nucleic Acids Res. 2020;48(22):12983-12999.
- Vien TN, Wang J, Ng LCT, Cao E, DeCaen PG. Molecular dysregulation of ciliary polycystin-2 channels caused by variants in the TOP domain. Proc Natl Acad Sci U S A. 2020;117(19):10329-10338.
- Wang C, Molodtsov V, Firlar E, Kaelber JT, Blaha G, Su M, Ebright RH. Structural basis of transcription-translation coupling. Science. 2020;369(6509):1359-1365.
- Yang X, Wang Q, Cao E. Structure of the human cation-chloride cotransporter NKCC1 determined by single-particle electron cryo-microscopy. Nat Commun. 2020;11(1):1016.
- Yoder N, Gouaux E. The His-Gly motif of acid-sensing ion channels resides in a reentrant ‘loop’ implicated in gating and ion selectivity. eLife. 2020;9:e56527.
- Zheng X, Fu Z, Su D, Zhang Y, Li M, Pan Y, Li H, Li S, Grassucci RA, Ren Z, Hu Z, Li X, Zhou M, Li G, Frank J, Yang J. Mechanism of ligand activation of a eukaryotic cyclic nucleotide-gated channel. Nat Struct Mol Biol. 2020;27(7):625-634.
- Zhou T, Teng IT, Olia AS, Cerutti G, Gorman J, Nazzari A, Shi W, Tsybovsky Y, Wang L, Wang S, Zhang B, Zhang Y, Katsamba PS, Petrova Y, Banach BB, Fahad AS, Liu L, Lopez Acevedo SN, Madan B, Oliveira de Souza M, Pan X, Wang P, Wolfe JR, Yin M, Ho DD, Phung E, DiPiazza A, Chang LA, Abiona OM, Corbett KS, DeKosky BJ, Graham BS, Mascola JR, Misasi J, Ruckwardt T, Sullivan NJ, Shapiro L, Kwong PD. Structure-Based Design with Tag-Based Purification and In-Process Biotinylation Enable Streamlined Development of SARS-CoV-2 Spike Molecular Probes. Cell reports. 2020;33(4):108322.
- Zhou T, Tsybovsky Y, Gorman J, Rapp M, Cerutti G, Chuang GY, Katsamba PS, Sampson JM, Schon A, Bimela J, Boyington JC, Nazzari A, Olia AS, Shi W, Sastry M, Stephens T, Stuckey J, Teng IT, Wang P, Wang S, Zhang B, Friesner RA, Ho DD, Mascola JR, Shapiro L, Kwong PD. Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains. Cell Host Microbe. 2020;28(6):867-879 e865.
Publications
2024
- Padi SKR, Vos MR, Godek RJ, Fuller JR, Kruse T, Hein JB, et al. Cryo-EM structures of PP2A:B55-FAM122A and PP2A:B55-ARPP19. Nature. 2024.
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Wang L, Hoang A, Gil-Iturbe E, Laganowsky A, Quick M, Zhou M. Mechanism of anion exchange and small-molecule inhibition of pendrin. Nat Commun. 2024.
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Zilberg G, Parpounas AK, Warren AL, Yang S, Wacker D. Molecular basis of human trace amine-associated receptor 1 activation. Nat Commun. 2024.
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Yang R, Ko YH, Li F, Lokareddy RK, Hou CD, Kim C, et al. Structural basis for nuclear import of hepatitis B virus (HBV) nucleocapsid core. Sci Adv. 2024.
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Sharma S, Luo M, Patel H, Mueller DM, Liao M. Conformational ensemble of yeast ATP synthase at low pH reveals unique intermediates and plasticity in F(1)-F(o) coupling. Nat Struct Mol Biol. 2024.
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Nguyen BA, Singh V, Afrin S, Yakubovska A, Wang L, Ahmed Y, et al. Structural polymorphism of amyloid fibrils in ATTR amyloidosis revealed by cryo-electron microscopy. Nat Commun. 2024.
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Baldwin ET, van Eeuwen T, Hoyos D, Zalevsky A, Tchesnokov EP, Sanchez R, et al. Structures, functions and adaptations of the human LINE-1 ORF2 protein. Nature. 2024.
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Decato D, Palatinus L, Stierle A, Stierle D. Absolute structure determination of Berkecoumarin by X-ray and electron diffraction. Acta Crystallogr C Struct Chem. 2024.
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Hou K, Ge P, Sawaya MR, Dolinsky JL, Yang Y, Jiang YX, et al. How short peptides can disassemble ultra-stable tau fibrils extracted from Alzheimer’s disease brain by a strain-relief mechanism. bioRxiv. 2024.
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Zilberg G, Parpounas AK, Warren AL, Fiorillo B, Provasi D, Filizola M, et al. Structural insights into the unexpected agonism of tetracyclic antidepressants at serotonin receptors 5-HT(1e)R and 5-HT(1F)R. Sci Adv. 2024.
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Zuo H, Park J, Frangaj A, Ye J, Lu G, Manning JJ, et al. Promiscuous G-protein activation by the calcium-sensing receptor. Nature. 2024.
2023
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Atanasoff KE, Brambilla L, Adelsberg DC, Kowdle S, Stevens CS, Hung CT, et al. An in vitro experimental pipeline to characterize the binding specificity of SARS-CoV-2 neutralizing antibodies. bioRxiv. 2023.
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Capper MJ, Yang S, Stone AC, Vatansever S, Zilberg G, Mathiharan YK, et al. Substrate binding and inhibition of the anion exchanger 1 transporter. Nat Struct Mol Biol. 2023.
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Consalvo CD, Aderounmu AM, Donelick HM, Aruscavage PJ, Eckert DM, Shen PS, et al. C. elegans Dicer acts with the RIG-I-like helicase DRH-1 and RDE-4 to cleave dsRNA. bioRxiv. 2023.
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Edman NI, Redler RL, Phal A, Schlichthaerle T, Srivatsan SR, Etemadi A, et al. Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assemblies. bioRxiv. 2023.
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Feng S, Aplin C, Nguyen TT, Cerione RA. Filament formation by glutaminase enzymes drives catalysis. bioRxiv. 2023.
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Frey SJ, Carreno JM, Bielak D, Arsiwala A, Altomare CG, Varner C, et al. Nanovaccines Displaying the Influenza Virus Hemagglutinin in an Inverted Orientation Elicit an Enhanced Stalk-Directed Antibody Response. Adv Healthc Mater. 2023.
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Gangwar SP, Yen LY, Yelshanskaya MV, Sobolevsky AI. Positive and negative allosteric modulation of GluK2 kainate receptors by BPAM344 and antiepileptic perampanel. Cell reports. 2023.
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Gibbs E, Klemm E, Seiferth D, Kumar A, Ilca SL, Biggin PC, et al. Conformational transitions and allosteric modulation in a heteromeric glycine receptor. Nat Commun. 2023.
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Han BG, Avila-Sakar A, Remis J, Glaeser RM. Challenges in making ideal cryo-EM samples. Curr Opin Struct Biol. 2023.
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Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, et al. Structural basis for breadth development in the HIV-1 V3-glycan targeting DH270 antibody clonal lineage. Nat Commun. 2023.
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Hoang Trinh TK, Catalano C, Guo Y. Fabrication of membrane proteins in the form of native cell membrane nanoparticles using novel membrane active polymers. Nanoscale Adv. 2023.
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Hu Z, Zheng X, Yang J. Conformational trajectory of allosteric gating of the human cone photoreceptor cyclic nucleotide-gated channel. Nat Commun. 2023.
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Huddy TF, Hsia Y, Kibler RD, Xu J, Bethel N, Nagarajan D, et al. Blueprinting expandable nanomaterials with standardized protein building blocks. bioRxiv. 2023.
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Kulczyk AW, Sorzano COS, Grela P, Tchorzewski M, Tumer NE, Li XP. Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk reveals residues involved in the binding interaction. J Biol Chem. 2023.
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Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, et al. Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry. Nat Commun. 2023.
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Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, et al. High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217. Nat Commun. 2023.
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Malone BF, Perry JK, Olinares PDB, Lee HW, Chen J, Appleby TC, et al. Structural basis for substrate selection by the SARS-CoV-2 replicase. Nature. 2023.
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Molodtsov V, Wang C, Firlar E, Kaelber JT, Ebright RH. Structural basis of Rho-dependent transcription termination. Nature. 2023.
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Monsen RC, Chua EYD, Hopkins JB, Chaires JB, Trent JO. Structure of a 28.5 kDa duplex-embedded G-quadruplex system resolved to 7.4 A resolution with cryo-EM. Nucleic Acids Res. 2023.
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Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, et al. A general mechanism for transcription bubble nucleation in bacteria. Proc Natl Acad Sci U S A. 2023.
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Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, et al. Structures of the holo CRISPR RNA-guided transposon integration complex. Nature. 2023.
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Patel A, Kumar S, Lai L, Chakravarthy C, Valanparambil R, Reddy ES, et al. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response. Structure. 2023.
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Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, et al. Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays. Nat Struct Mol Biol. 2023.
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Wang C, Molodtsov V, Sanders TJ, Marshall CJ, Firlar E, Kaelber JT, et al. Structural basis of archaeal FttA-dependent transcription termination. bioRxiv. 2023.
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Watkins MB, Wang H, Burnim A, Ando N. Conformational switching and flexibility in cobalamin-dependent methionine synthase studied by small-angle X-ray scattering and cryo-electron microscopy. bioRxiv. 2023.
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Weaver JW, Proshkin S, Duan W, Epshtein V, Gowder M, Bharati BK, et al. Control of transcription elongation and DNA repair by alarmone ppGpp. Nat Struct Mol Biol. 2023.
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Wilbon AS, Shen J, Ruchala P, Zhou M, Pan Y. Structural basis of ferroportin inhibition by minihepcidin PR73. PLoS Biol. 2023.
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Yang Y, Fritzsching KJ, He S, McDermott AE. Zinc Alters the Supramolecular Organization of Nucleic Acid Complexes with Full-Length TIA1. bioRxiv. 2023.
2022
- Capper MJ, Yang S, Stone AC, Vatansever S, Zilberg G, Mathiharan YK, Habib R, Hutchinson K, Schlessinger A, Mezei M, Osman R, Zhang B, Wacker D. Substrate Binding and Inhibition of the Anion Exchanger 1 Transporter. bioRxiv. 2022. [preprint].
- Chen J, Wang Q, Malone B, Llewellyn E, Pechersky Y, Maruthi K, Eng ET, Perry JK, Campbell EA, Shaw DE, Darst SA. Ensemble cryo-EM reveals conformational states of the nsp13 helicase in the SARS-CoV-2 helicase replication-transcription complex. Nat Struct Mol Biol. 2022;29(3):250-260.
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Finkelstein MT, Parker Miller E, Erdman MC, Fera D. Analysis of two cooperating antibodies unveils immune pressure imposed on HIV Env to elicit a V3-glycan supersite broadly neutralizing antibody lineage. Front Immunol. 2022.
- Gobeil SMC, Henderson R, Stalls V, Janowska K, Huang X, May A, Speakman M, Beaudoin E, Manne K, Li D, Parks R, Barr M, Deyton M, Martin M, Mansouri K, Edwards RJ, Sempowski GD, Saunders KO, Wiehe K, Williams W, Korber B, Haynes BF, Acharya P. Structural diversity of the SARS-CoV-2 Omicron spike. bioRxiv. 2022.
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Henderson R, Zhou Y, Stalls V, Wiehe K, Saunders KO, Wagh K, et al. Structural basis for breadth development in a HIV-1 neutralizing antibody. bioRxiv. 2022.
- Kendall AK, Chandra M, Xie B, Wan W, Jackson LP. Improved mammalian retromer cryo-EM structures reveal a new assembly interface. bioRxiv. 2022.
- Klykov O, Kopylov M, Carragher B, Heck AJR, Noble AJ, Scheltema RA. Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology. Mol Cell. 2022.
- Koo CW, Tucci FJ, He Y, Rosenzweig AC. Recovery of particulate methane monooxygenase structure and activity in a lipid bilayer. Science. 2022.
- Laughlin ZT, Nandi S, Dey D, Zelinskaya N, Witek MA, Srinivas P, Nguyen HA, Kuiper EG, Comstock LR, Dunham CM, Conn GL. 50S subunit recognition and modification by the Mycobacterium tuberculosis ribosomal RNA methyltransferase TlyA. Proc Natl Acad Sci U S A. 2022.
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Leonhardt SA, Purdy MD, Grover JR, Yang Z, Poulos S, McIntire WE, et al. CryoEM Structures of the Human HIV-1 Restriction Factor SERINC3 and Function as a Lipid Transporter. bioRxiv. 2022.
- Liang WG, Wijaya J, Wei H, Noble AJ, Mancl JM, Mo S, Lee D, Lin King JV, Pan M, Liu C, Koehler CM, Zhao M, Potter CS, Carragher B, Li S, Tang WJ. Structural basis for the mechanisms of human presequence protease conformational switch and substrate recognition. Nat Commun. 2022.
- Malone BF, Perry JK, Olinares PDB, Chen J, Appelby TK, Feng JY, Bilello JP, Ng H, Sotiris J, Ebrahim M, Chua EYD, Mendez JH, Eng ET, Landick R, Chait BT, Campbell EA, Darst SA. Structural basis for substrate selection by the SARS-CoV-2 replicase. bioRxiv. 2022.
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Nguyen BA, Afrin S, Singh V, Ahmed Y, Pedretti R, del Carmen Fernandez-Ramirez M, et al. Structural polymorphism of amyloid fibrils in cardiac ATTR amyloidosis revealed by cryo-electron microscopy. bioRxiv. 2022.
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Patel A, Kumar S, Lai L, Chakravarthy C, Valanparambil R, Reddy ES, et al. Molecular basis of SARS-CoV-2 Omicron variant evasion from shared neutralizing antibody response. bioRxiv. 2022.
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Smith EM, Ferrell S, Tokars VL, Mondragon A. Structures of an active type III-A CRISPR effector complex. Structure. 2022.
- Trinh TKH, Catalano C, Guo Y. Membrane-active Polymers: NCMNP13-x, NCMNP21-x and NCMNP21b-x for Membrane Protein Structural Biology. bioRxiv. 2022.
- Tsai K, Stojkovic V, Lee DJ, Young ID, Szal T, Klepacki D, Vazquez-Laslop N, Mankin AS, Fraser JS, Fujimori DG. Structural basis for context-specific inhibition of translation by oxazolidinone antibiotics. Nat Struct Mol Biol. 2022.
- Wasmuth EV, Broeck AV, LaClair JR, Hoover EA, Lawrence KE, Paknejad N, Pappas K, Matthies D, Wang B, Feng W, Watson PA, Zinder JC, Karthaus WR, de la Cruz MJ, Hite RK, Manova-Todorova K, Yu Z, Weintraub ST, Klinge S, Sawyers CL. Allosteric interactions prime androgen receptor dimerization and activation. bioRxiv. 2022. [preprint].
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Wilbon AS, Shen J, Ruchala P, Zhou M, Pan Y. Structural basis of ferroportin inhibition by minihepcidin PR73. bioRxiv. 2022.
- Yelshanskaya MV, Patel DS, Kottke CM, Kurnikova MG, Sobolevsky AI. Opening of glutamate receptor channel to subconductance levels. Nature. 2022.
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Yin Y, Zhang F, Feng S, Butay KJ, Borgnia MJ, Im W, et al. Activation mechanism of the mouse cold-sensing TRPM8 channel by cooling agonist and PIP(2). Science. 2022.
- Yin Z, Bird JG, Kaelber JT, Nickels BE, Ebright RH. Structural basis of transcription antitermination by Qλ: NusA induces refolding of Qλ to form nozzle for RNA polymerase exit channel. bioRxiv. 2022. [preprint]
- Yu Y, Li S, Ser Z, Kuang H, Than T, Guan D, Zhao X, Patel DJ. Cryo-EM structure of DNA-bound Smc5/6 reveals DNA clamping enabled by multi-subunit conformational changes. Proc Natl Acad Sci U S A. 2022.
- Zhao Y, Roy K, Vidossich P, Cancedda L, De Vivo M, Forbush B, Cao E. Structural basis for inhibition of the Cation-chloride cotransporter NKCC1 by the diuretic drug bumetanide. Nat Commun. 2022;13(1):2747.
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Zhao Y, Shen J, Wang Q, Ruiz Munevar MJ, Vidossich P, De Vivo M, et al. Structure of the human cation-chloride cotransport KCC1 in an outward-open state. Proc Natl Acad Sci U S A. 2022.
- Zheng X, Hu Z, Li H, Yang J. Structure of the human cone photoreceptor cyclic nucleotide-gated channel. Nat Struct Mol Biol. 2022;29(1):40-46.
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Zhou W, Yadav GP, Yang X, Qin F, Li C, Jiang QX. Cryo-EM structure-based selection of computed ligand poses enables design of MTA-synergic PRMT5 inhibitors of better potency. Commun Biol. 2022.
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Zhu J, Huang W, Zhao J, Huynh L, Taylor DJ, Harris ME. Structural and mechanistic basis for recognition of alternative tRNA precursor substrates by bacterial ribonuclease P. Nat Commun. 2022.
- Zimanyi CM, Kopylov M, Potter CS, Carragher B, Eng ET. Broadening access to cryoEM through centralized facilities. Trends Biochem Sci. 2022.
2021
- Catalano C, Ben-Hail D, Qiu W, Blount P, des Georges A, Guo Y. Cryo-EM Structure of Mechanosensitive Channel YnaI Using SMA2000: Challenges and Opportunities. Membranes. 2021;11(11):849.
- Cerutti G, Guo Y, Zhou T, Gorman J, Lee M, Rapp M, Reddem ER, Yu J, Bahna F, Bimela J, Huang Y, Katsamba PS, Liu L, Nair MS, Rawi R, Olia AS, Wang P, Zhang B, Chuang GY, Ho DD, Sheng Z, Kwong PD, Shapiro L. Potent SARS-CoV-2 neutralizing antibodies directed against spike N-terminal domain target a single supersite. Cell Host Microbe. 2021:2021.2001.2010.426120.
- Cerutti G, Rapp M, Guo Y, Bahna F, Bimela J, Reddem ER, Yu J, Wang P, Liu L, Huang Y, Ho DD, Kwong PD, Sheng Z, Shapiro L. Structural basis for accommodation of emerging B.1.351 and B.1.1.7 variants by two potent SARS-CoV-2 neutralizing antibodies. Structure. 2021;29(7):655-663 e654.
- Chen K-E, Guo Q, Cui Y, Kendall AK, Hill TA, Hall RJ, Sacharz J, Norwood SJ, Xie B, Leneva N, Yang Z, Ghai R, Stroud DA, Fairlie D, Suga H, Jackson LP, Teasdale RD, Passioura T, Collins BM. De novo macrocyclic peptides for inhibiting, stabilising and probing the function of the Retromer endosomal trafficking complex. bioRxiv. 2021. [preprint]
- Feldman J, Bals J, Altomare CG, St Denis K, Lam EC, Hauser BM, Ronsard L, Sangesland M, Bracamonte Moreno T, Okonkwo V, Hartojo N, Balazs AB, Bajic G, Lingwood D, Schmidt AG. Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses. Sci Immunol. 2021:eabl5842.
- Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Manne K, Stalls V, Kopp MF, Henderson R, Edwards RJ, Haynes BF, Acharya P. D614G Mutation Alters SARS-CoV-2 Spike Conformation and Enhances Protease Cleavage at the S1/S2 Junction. Cell reports. 2021;34(2):108630.
- Gobeil SM, Janowska K, McDowell S, Mansouri K, Parks R, Stalls V, Kopp MF, Manne K, Li D, Wiehe K, Saunders KO, Edwards RJ, Korber B, Haynes BF, Henderson R, Acharya P. Effect of natural mutations of SARS-CoV-2 on spike structure, conformation, and antigenicity. Science. 2021;373(6555).
- He Y, Wang Y, Liu B, Helmling C, Sušac L, Cheng R, Zhou ZH, Feigon J. Structures of telomerase at several steps of telomere repeat synthesis. Nature. 2021;593(7859):454-459.
- Herrera NG, Morano NC, Celikgil A, Georgiev GI, Malonis RJ, Lee JH, Tong K, Vergnolle O, Massimi AB, Yen LY, Noble AJ, Kopylov M, Bonanno JB, Garrett-Thomson SC, Hayes DB, Bortz RH, 3rd, Wirchnianski AS, Florez C, Laudermilch E, Haslwanter D, Fels JM, Dieterle ME, Jangra RK, Barnhill J, Mengotto A, Kimmel D, Daily JP, Pirofski LA, Chandran K, Brenowitz M, Garforth SJ, Eng ET, Lai JR, Almo SC. Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis. ACS Omega. 2021;6(1):85-102.
- Klykov O, Gangwar SP, Yelshanskaya MV, Yen L, Sobolevsky AI. Structure and desensitization of AMPA receptor complexes with type II TARP gamma5 and GSG1L. Mol Cell. 2021.
- Kumar P, Cymes GD, Grosman C. Structure and function at the lipid-protein interface of a pentameric ligand-gated ion channel. Proc Natl Acad Sci U S A. 2021;118(23):e2100164118.
- Li D, Edwards RJ, Manne K, Martinez DR, Schafer A, Alam SM, Wiehe K, Lu X, Parks R, Sutherland LL, Oguin TH, 3rd, McDanal C, Perez LG, Mansouri K, Gobeil SMC, Janowska K, Stalls V, Kopp M, Cai F, Lee E, Foulger A, Hernandez GE, Sanzone A, Tilahun K, Jiang C, Tse LV, Bock KW, Minai M, Nagata BM, Cronin K, Gee-Lai V, Deyton M, Barr M, Von Holle T, Macintyre AN, Stover E, Feldman J, Hauser BM, Caradonna TM, Scobey TD, Rountree W, Wang Y, Moody MA, Cain DW, DeMarco CT, Denny TN, Woods CW, Petzold EW, Schmidt AG, Teng IT, Zhou T, Kwong PD, Mascola JR, Graham BS, Moore IN, Seder R, Andersen H, Lewis MG, Montefiori DC, Sempowski GD, Baric RS, Acharya P, Haynes BF, Saunders KO. In vitro and in vivo functions of SARS-CoV-2 infection-enhancing and neutralizing antibodies. Cell. 2021.
- Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA. Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex. Proc Natl Acad Sci U S A. 2021;118(19).
- Moghadamchargari Z, Shirzadeh M, Liu C, Schrecke S, Packianathan C, Russell DH, Zhao M, Laganowsky A. Molecular assemblies of the catalytic domain of SOS with KRas and oncogenic mutants. Proc Natl Acad Sci U S A. 2021;118(12).
- Nadezhdin KD, Neuberger A, Nikolaev YA, Murphy LA, Gracheva EO, Bagriantsev SN, Sobolevsky AI. Extracellular cap domain is an essential component of the TRPV1 gating mechanism. Nat Commun. 2021;12(1):2154.
- Olinares PDB, Kang JY, Llewellyn E, Chiu C, Chen J, Malone B, Saecker RM, Campbell EA, Darst SA, Chait BT. Native Mass Spectrometry-Based Screening for Optimal Sample Preparation in Single-Particle Cryo-EM. Structure. 2021;29(2):186-195 e186.
- Nadezhdin KD, Neuberger A, Trofimov YA, Krylov NA, Sinica V, Kupko N, Vlachova V, Zakharian E, Efremov RG, Sobolevsky AI. Structural mechanism of heat-induced opening of a temperature-sensitive TRP channel. Nat Struct Mol Biol. 2021;28(7):564-572.
- Rangarajan ES, Izard T. The Cryogenic Electron Microscopy Structure of the Cell Adhesion Regulator Metavinculin Reveals an Isoform-Specific Kinked Helix in Its Cytoskeleton Binding Domain. Int J Mol Sci. 2021;22(2).
- Rapp M, Guo Y, Reddem ER, Yu J, Liu L, Wang P, Cerutti G, Katsamba P, Bimela JS, Bahna FA, Mannepalli SM, Zhang B, Kwong PD, Huang Y, Ho DD, Shapiro L, Sheng Z. Modular basis for potent SARS-CoV-2 neutralization by a prevalent VH1-2-derived antibody class. Cell reports. 2021;35(1):108950.
- Sauer DB, Wang B, Sudar JC, Song J, Marden J, Rice WJ, Wang DN. The ups and downs of elevator-type di-/tricarboxylate membrane transporters. FEBS J. 2021.
- Schmitz AJ, Turner JS, Liu Z, Zhou JQ, Aziati ID, Chen RE, Joshi A, Bricker TL, Darling TL, Adelsberg DC, Altomare CG, Alsoussi WB, Case JB, VanBlargan LA, Lei T, Thapa M, Amanat F, Jeevan T, Fabrizio T, O’Halloran JA, Shi PY, Presti RM, Webby RJ, Krammer F, Whelan SPJ, Bajic G, Diamond MS, Boon ACM, Ellebedy AH. A vaccine-induced public antibody protects against SARS-CoV-2 and emerging variants. Immunity. 2021;54(9):2159-2166 e2156.
- Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H. Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment. Immunity. 2021.
- Sun D, Sang Z, Kim YJ, Xiang Y, Cohen T, Belford AK, Huet A, Conway JF, Sun J, Taylor DJ, Schneidman-Duhovny D, Zhang C, Huang W, Shi Y. Potent neutralizing nanobodies resist convergent circulating variants of SARS-CoV-2 by targeting diverse and conserved epitopes. Nat Commun. 2021;12(1):4676.
- van Eeuwen T, Shim Y, Kim HJ, Zhao T, Basu S, Garcia BA, Kaplan CD, Min JH, Murakami K. Cryo-EM structure of TFIIH/Rad4-Rad23-Rad33 in damaged DNA opening in nucleotide excision repair. Nat Commun. 2021;12(1):3338.
- Wang JC, Chen L. Structural basis for the structural dynamics of human mitochondrial chaperonin mHsp60. Sci Rep. 2021;11(1):14809.
- Williams WB, Meyerhoff RR, Edwards RJ, Li H, Manne K, Nicely NI, Henderson R, Zhou Y, Janowska K, Mansouri K, Gobeil S, Evangelous T, Hora B, Berry M, Abuahmad AY, Sprenz J, Deyton M, Stalls V, Kopp M, Hsu AL, Borgnia MJ, Stewart-Jones GBE, Lee MS, Bronkema N, Moody MA, Wiehe K, Bradley T, Alam SM, Parks RJ, Foulger A, Oguin T, Sempowski GD, Bonsignori M, LaBranche CC, Montefiori DC, Seaman M, Santra S, Perfect J, Francica JR, Lynn GM, Aussedat B, Walkowicz WE, Laga R, Kelsoe G, Saunders KO, Fera D, Kwong PD, Seder RA, Bartesaghi A, Shaw GM, Acharya P, Haynes BF. Fab-dimerized glycan-reactive antibodies are a structural category of natural antibodies. Cell. 2021;184(11):2955-2972 e2925.
- Yang D, Gouaux E. Illumination of serotonin transporter mechanism and role of the allosteric site. Sci Adv. 2021;7(49):eabl3857.
2020
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- Santosh V, Musayev FN, Jaiswal R, Zarate-Perez F, Vandewinkel B, Dierckx C, Endicott M, Sharifi K, Dryden K, Henckaerts E, Escalante CR. The Cryo-EM structure of AAV2 Rep68 in complex with ssDNA reveals a malleable AAA+ machine that can switch between oligomeric states. Nucleic Acids Res. 2020;48(22):12983-12999.
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- Yoder N, Gouaux E. The His-Gly motif of acid-sensing ion channels resides in a reentrant ‘loop’ implicated in gating and ion selectivity. eLife. 2020;9:e56527.
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