• 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • April 10-14, 2023.

  • At no cost.

  • Room and board NOT included.

Apply Here.

To join the waitlist or for more information email nccatuseroffice [at] nysbc.org

see the 2021 shortcourse

Tomography Short Course
April 10-14, 2023

NCITU & NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. Cryo-electron tomography (cryo-ET) is still emerging as a technique for directly visualizing macromolecular associations and organization in native cells. This course will step through each stage of the cryo-ET workflow (specimen preparation, data acquisition, computational processing, and national infrastructure) and present methodologies that participating trainees could implement at their home institutions. The course curriculum will cover cutting-edge methods in cryo-ET and help attendees accelerate their research with exposure to these methods. The mornings involve lectures and stimulating round table discussions. The afternoons offer hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day. Users are welcome to bring their own samples and/or data.

Short-course Agenda

Monday, April 10, 2023

Session Type Lecturer Topic
Morning Lecture 1 – Keynote TBD Intro and overview of tomography
Morning Roundtable 1 students Flashtalks from students
Afternoon Lecture 2 TBD Choosing the right in situ target and model system
Afternoon Practical 1 SEMC staff Vitrification of cells and tissues practicals

Tuesday, April 11, 2023

Session Type Lecturer Topic
Morning Lecture 3 SEMC staff FIB-SEM & Dual beams
Afternoon Roundtable 2 SEMC staff Methodologies for making windows into cells
Afternoon Practical 2 SEMC staff cryoCLEM and cryoFIB practicals

Wednesday, April 12, 2023

Session Type Lecturer Topic
Morning Lecture 4 TBD Cryo-Applications and data collection
Morning Roundtable 3 TBD New software and algorithm development
Afternoon Practical 3 SEMC staff cryoET data collection practical
Afternoon Practical 2 SEMC staff additional time on dual beams

Thursday, April 13, 2023

Session Type Lecturer Topic
Morning Lecture 6 TBD Automated reconstruction, subtomogram averaging methods & data interpretation
Afternoon Practical 4 TBD Data processing practical
Lunch Roundtable 4 TBD EM challenges and new frontiers
Afternoon Practical 4 SEMC staff The future of in situ cellular structural biology
Dinner all instructors Group dinner

Friday, April 14, 2023

Session Type Lecturer Topic
Morning Roundtable 5 TBD The future of in situ cellular structural biology .
Afternoon Practical 5 TBD Annotation software : EMAN2 and Amira

This workshop is offered at NO COST, but limited to 16 attendees.

Apply today.

We will reach out to participants by end of January to the beginning of February. For further inquires please email the NCCAT User Office.

Yes. This will be an in-person on-site workshop. All attendees should be prepared to provide a CDC vaccination card or show their New York State Excelsior Pass showing proof of COVID-19 vaccination.

A copy of the NYSBC’s COVID-19 policies may be found here.

Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.