• 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • April 10-14, 2023.

  • At no cost.

  • Room and board NOT included.

Apply Here.

To join the waitlist or for more information email nccatuseroffice [at] nysbc.org

see the 2021 shortcourse

Tomography Short Course
April 10-14, 2023

NCITU & NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. Cryo-electron tomography (cryo-ET) is still emerging as a technique for directly visualizing macromolecular associations and organization in native cells. This course will step through each stage of the cryo-ET workflow (specimen preparation, data acquisition, computational processing, and national infrastructure) and present methodologies that participating trainees could implement at their home institutions. The course curriculum will cover cutting-edge methods in cryo-ET and help attendees accelerate their research with exposure to these methods. The mornings involve lectures and stimulating round table discussions. The afternoons offer hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day. Users are welcome to bring their own samples and/or data.

Short-course Agenda

Monday, April 10, 2023

Session Type Lecturer Topic
Morning Lecture 1 NCITU/NCCAT staff Intro and overview of tomography
Morning Roundtable 1 students Flash talks from students
Afternoon Roundtable 2 NCITU/NCCAT staff Choosing the right in situ target and model system
Afternoon Practical 1 NCITU/NCCAT staff Vitrification of cells and tissues practicals (plunge freezing, HPF and PRIMO)

Tuesday, April 11, 2023

Session Type Lecturer Topic
Morning Lecture 2 NCITU/NCCAT staff Introduction to FIB-SEM & Dual beams
Afternoon Roundtable 3 NCITU/NCCAT staff Methodologies for making windows into cells
Afternoon Practical 2 NCITU/NCCAT staff cryoCLEM and cryoFIB practicals (Airyscan, Helios and Aquilos2)

Wednesday, April 12, 2023

Session Type Lecturer Topic
Morning Lecture 3 NCITU/NCCAT staff Cryo-Applications and data collection on autoloader systems
Morning Roundtable 4 NCITU/NCCAT staff New software and algorithm development
Afternoon Practical 3 NCITU/NCCAT staff cryoET data collection practical (Glacios and Krios)
Afternoon Practical 2b NCITU/NCCAT staff additional time on dual beams

Thursday, April 13, 2023

Session Type Lecturer Topic
Morning Lecture 4 NCITU/NCCAT staff Automated reconstruction, subtomogram averaging methods & data interpretation
Afternoon Practical 4 NCITU/NCCAT staff Data processing practical
Lunch Roundtable 5 NCITU/NCCAT staff EM challenges and new frontiers
Afternoon Practical 3b NCITU/NCCAT staff additional time for cryoET data collection
Dinner all attendees Group dinner

Friday, April 14, 2023

Session Type Lecturer Topic
Morning Roundtable 6 NCITU/NCCAT staff The future of in situ cellular structural biology .
Morning Practical 5 NCITU/NCCAT staff Annotation software : EMAN2 and Amira
Afternoon Practical 3c NCITU/NCCAT staff additional time for cryoET data collection
Afternoon all attendees farewell

This workshop is offered at NO COST, but is limited to 8 attendees.
Attendees are encouraged to bring samples and data.

Apply today.

We will reach out to participants by end of January to confirm acceptance into the short course. For further inquiries please email the NCCAT User Office.

Yes. This will be an in-person on-site workshop. All attendees should be prepared to provide a CDC vaccination card or show their New York State Excelsior Pass showing proof of COVID-19 vaccination.

A copy of the NYSBC’s COVID-19 policies may be found here.

Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.