SEMC and NCCAT will be offering a 3-day workshop focused on the theory and practice of tomographic methods. Cryoelectron tomography (cryoET) is a powerful new method for directly visualizing macromolecular associations and organization in native cells Traditionally, macromolecular structure determination has been limited to the analysis of isolated molecules and structures. CryoET has the unique ability to visualize macromolecular organization in native cells; it utilizes the intrinsic contrast of frozen cellular material to directly image macromolecules.
13:00-13:30 Full Overview – Tilt-Series Collection, Appion-Protomo alignment, and Sub-tomogram Processing
13:30-14:00 In-Depth Appion-Protomo Workflow
14:00-14:30 Q&A and Practice!
15:00-15:10 Overview – Sub-Tomogram Alignment and Dynamo
15:10-15:35 Particle Picking using a Template
15:35-16:00 Membrane, and Filament Picking
16:00-16:30 Sub-Tomogram Alignment, Classification, and tools to transfer to Warp/M/Relion
16:30-17:00 Q&A and Practice!
April 14, 2021
Day 3: Practical – EMAN2 (limited space available)
Practical pre-requisite – Computational setup for workshop[AWS startup pdf]
EMAN2 tools, sub-tomogram averaging and annotation Muyuan Chen(Baylor College of Medicine) [tutorial information] recording: [EMAN2]
12:05-12:50 Basic user interface and visualization of raw data.
12:50-13:35 Tilt series alignment, tomogram reconstruction and CTF estimation
13:35-14:30 Methods for picking particles from tomograms
15:00-16:15 Generating initial model and setting up subtomogram averaging runs