ACA Workshop 5: CryoEM sample preparation training using center merit badges
Held during the 72nd ACA Annual Meeting | July 29 – August 3, 2022
Cryo-electron microscopy (cryoEM) is a method used to determine high-resolution, three-dimensional structures of samples that could not be used with other techniques in the past, such as samples containing mixtures of different biological structures or intact cells. CryoEM instrumentation is being installed in many research institutions and there is a need to learn the best practices available in the field. This workshop was held on Friday, July 29, 2022, and focused on cryoEM educational resources available to researchers and provided hands-on training with cryoEM sample preparation instrumentation.
|Time (PDT)||Instructor / Topic|
|8:30||Meet in the ACA lobby (look for people holding Workshop 5 sign).|
|8:35-9:00||Group escorted to PNCC location.|
|9:00-9:05||Craig Yoshioka, OHSU & Ed Eng, NYSBC – Welcome and introduction to NIH Transformative High-Resolution CryoEM Program – https://www.cryoemcenters.org/.|
|9:05-10:00||CryoEM Curriculum Development Part I (Moderated by Michael Schmid, S2C2).|
|9:05-9:30||Peter Shen, University of Utah – cryoEM101: How to use media and data visualization to aid the training efforts of newcomers to the field.|
|9:35-10:00||Wen Jiang & Yingjie Victor Chen, Purdue University – cryoVR: How Virtual Reality can be used to familiarize users with cryoEM equipment.|
|10:15-12:30pm||cryoEM Merit badges Part I.|
|10:15-10:30||Ed Eng, NYSBC & Claudia López, OHSU – Introduction to cryoEM Merit Badges and practical stations.|
|10:30-12:00||Students at their 1st & 2nd practical stations (must pre-register and rank top 5 choices).
|12:00-12:45pm||Lunch and discussion.|
|12:45-1:30pm||CryoEM Curriculum Development Part II.|
|12:45-1:10pm||Michael Cianfrocco, University of Michigan – cryoEDU: How to learn and practice cryoEM processing with limited computational resources.|
|1:10-1:30pm||Roundtable and general Q&A (Moderated by Michael Schmid, S2C2 and Claudia López, OHSU).|
|1:30-4:10pm||cryoEM Merit badges Part II.
Students rotate through 3rd, 4th and 5th practical stations (must pre-register and rank top 5 choices).
|4:10pm||Wrap-up and merit badge certification awards.|
|4:10pm-4:30pm||Return to the ACA Meeting Location.|
|4:30pm-6pm||Joint reception with WK-4, WK-3, and cryoEM SIG in Salons G, H & I.|
The objective of this workshop was to learn how to interface with the NIH-sponsored Transformative High-Resolution CryoEM Program, which offers a comprehensive online cryoEM curriculum that familiarizes new users with cryoEM equipment and workflow. Lectures and practicals will focus on everything from how to use the national cryoEM service centers, media-rich curriculum to augment users’ own hands-on training, and earning cryoEM merit badges. CryoEM proficiency badges are awarded to users in three main skill areas (Sample preparation, Microscope operations and Data processing). This workshop, in particular, focused on the “Sample preparation” merit badges. After trainees complete a necessary set of requirements, they may apply for a merit badge. Receipt of a merit badge will qualify the user for independence, and be cross-honored at other national service centers for instrumentation use.
This workshop was held off-site from the ACA meeting at PNCC which is a 15 min walk or 10 min Tri-Met trip from the Portland Marriott Downtown Waterfront, 1401 SW Naito Pkwy, Portland, OR 97201
Directions to workshop site:
Robertson Life Sciences Building
2730 S. Moody Ave
Portland, OR 97201-5042
Yes. This was an in-person on-site workshop at PNCC in Portland, OR.
This workshop will be part of the main program of the ACA meeting, but held offsite at PNCC where the cryoEM instrumentation and laboratory space will be available. Due to OHSU’s COVID-19 Immunizations and Education policy, everyone visiting OHSU will need to provide proof of full COVID-19 vaccination or attestation from their home institution that they are compliant with OHSU and Oregon Health Authority vaccination policies (https://news.ohsu.edu/2021/08/04/ohsu-covid-19-vaccination-requirement-update) . For those with medical or religious exemption approvals, a letter from their home institutions will be required. Completing the ACA CrowdPass vaccination verification will be compliant with these policies.
The workshop will be in-person only, but we will post all learning materials on our website. No recordings were made during the workshop.