Knowledge of the workflow

Safety

Specific safety SOPs for this merit badge.

• NYSBC SOP for working with negative stains

For general safety information see the SOPs in the right bar.

Vendor product information

Chemicals for Electron Microscopy

• EMS Diasum
• Ted Pella
• SPI

Theoretical knowledge

Curriculum development partner modules

• CryoEM101 – Chapter 1: Sample purification [Section 5: Negative stain TEM]

Useful papers

• Negative Staining and Image Classification – Powerful Tools in Modern Electron Microscopy

Center specific guidelines

• NCCAT SOP for Negative Stain EM

A pass/fail test

The knowledge assessment test primarily covering a safety quiz will be given and may be updated regularly. If a user has earned the merit badge and policies have been updated, then an updated information sheet would have to be reviewed ahead of doing cryogenic work at the center.

• MB1: Negative Stain Quiz

Center staff training

The workflow demonstration should be scheduled with center staff through the NCCAT User Office or your appointed user coordinator.

Supervised and unsupervised practice by the user

Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.

Two (2) separate center staff observing your proficiency at separate times

Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Appointments may be made through the NCCAT User Office.