NCCAT & NRAMM
Workshop on graphene grids for cryoEM
March 25, 2020 | 9am-5pm
Due to COVID-19 this workshop will be postponed until further notice. We will keep you informed about updates after we revise our workshop agenda. We are currently discussing alternatives including broadcasting the lecture and also making a detailed recording of the entire process to share as a video.
Accessible and robust methods for preparing high-yield monolayer graphene grids for cryo-EM could significantly alleviate issues with grid preparation. The graphene grid making approach employed and published recently (Han, Fan et al. 2020) may increase particle concentration over grid holes without compromising high resolution structural information.
Authors of (Han, Fan et al., 2020) from Princeton University, in conjunction with the NCCAT and NRAMM, will lead a hands-on workshop aimed at teaching attendees how they make graphene grids for cryo-EM. With this knowledge, researchers will be able to prepare graphene grids in a typical biology lab using commercially available graphene sheets.
This workshop is offered at NO COST, but limited to 20 attendees.
If you already registered for the workshop, then we have your email on file for updates.
The workshop will be held on Wednesday, March 25, 2020 from 9am-5pm at NCCAT and NRAMM, which is located at the New York Structural Biology Center in NYC. Lunch will be served.
Workshop attendees are encouraged to bring cryoEM samples.
No previous experience in graphene grids is required.
During registration please note any dietary restrictions.
Single particle cryogenic electron microscopy (cryoEM) is a cutting-edge technology to determine three-dimensional atomic structures of bio-macromolecules. However, cryo-sample preparation issues may limit cryoEM from achieving higher resolution. Yimo and Xiao have established a method to obtain high yield, monolayer graphene support films to improve cryo-sample quality.