• 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • March 2-6, 2020.

  • At no cost.

  • Room and board NOT included.

Questions? Email nccatinfo [at] nysbc.org.

Sponsors

Single Particle Short Course
March 2-6, 2020

NCCAT offered a 1 week workshop focused on the theory and practice of single-particle analysis. The mornings were filled with lectures and stimulating round table discussions. The afternoons provided hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.

Short-course Agenda

Day 1: Monday, March 2, 2020

09:00 – 09:30 Registration and Welcome – SEMC conference room
09:50 – 10:00 Opening – NYSBC conference room
10:00 – 11:15 Keynote / Lecture 1 – NYSBC conference room
Intro and overview of SPA
Joachim Frank (Columbia University)
 [lecture slides][lecture video]
11:15 – 11:30 Break
11:30 – 12:15 Roundtable 1 – SEMC conference room
Introductions and course overview
Short course attendees
 [roundtable slides]
12:15 – 13:30 Lunch
13:30 – 14:15 Lecture 2 – SEMC conference room
cryoVR for sample preparation
Brenda Gonzalez & Jiahui Dong (Purdue University)
 [lecture slides][lecture video]
14:15 – 14:30 Break
14:30 – 18:00

14:30 – 18:00

Practical 1 – SEMC conference room
Support films and grids
Practical 2 – SEMC conference room
Plunge freezers and Chameleon
SEMC/NCCAT Staff
 [schedule]

Day 2: Tuesday, March 3, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
09:45 – 10:45 Lecture 3 – NYSBC conference room
Microscopes and tools of the trade
Ed Eng (NCCAT/NYSBC)
 [lecture slides][lecture video]
10:45 – 11:00 Break
11:00 – 12:00 Roundtable 2 – SEMC conference room
cryoEM Facility building
SEMC/NCCAT Staff
 [roundtable slides][roundtable video]
12:00 – 13:30 Lunch
13:30 – 17:30 Practical 3 – SEMC conference room
TEM microscopes
SEMC/NCCAT Staff
 [schedule]

Day 3: Wednesday, March 4, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
09:45 – 10:35

10:40 – 11:30

Lecture 4 – NYSBC conference room
Algorithms and foundational math PartI:
     derivation/explanation of the CTF, FTs.
Algorithms and foundational math PartII:
reconstruction, classification, maximum likelihood.
Fred Sigworth (Yale University)
 [lecture slides 1 lecture slides 2 lecture slides 3 lecture slides 4]
 [lecture notes SPR lecture notes reconstruction lecture notes fourier2D-3D lecture notes fourier1D lecture notes phase contrast lecture notes complex numbers]

 [lecture video]
11:30 – 11:45 Break
11:45 – 12:30 Lecture 5 – NYSBC conference room
Data Analysis and reconstruction workflow
Amedee des Georges (ASRC/CUNY)
 [lecture slides][lecture video]
12:30 – 13:15 Lunch & Roundtable 3 – NYSBC conference room
Current algorithms to deal with SPA reconstruction
Fred Sigworth, Amedee des Georges
 [roundtable video]
13:15 – 13:30 Group photo
13:30 – 17:00

13:30 – 17:00
by appointment

Practical 4 – SEMC conference room
CryoEM on Demand (processing in the cloud using AWS)
Practical 3B – Krios control room
TEM microscopes (continued) – Krios operations
SEMC/NCCAT Staff

Day 4: Thursday, March 5, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
09:45 – 10:45 Lecture 6 – NYSBC conference room
Interpretation and Limitations of SPA
Rich Hite (Memorial Sloan Kettering Cancer Center)
 [lecture slides][lecture video]
10:45 – 11:45 Lecture 7 – NYSBC conference room
Validation Methods
Tom Walz (Rockefeller University)
 [lecture slides][lecture video]
11:45 – 12:15 Roundtable 4 – NYSBC conference room
EM challenges and new frontiers
Rich Hite, Tom Walz
 [rountable video]
12:15 – 13:30 Lunch
13:30 – 15:45 Practical 4B – SEMC conference room
CryoEM on Demand (wrap-up)
mini-roundtable: Results, resolution and EM maps
SEMC/NCCAT Staff
 [summary slide]
13:30 – 17:00
by appointment
Practical 3C – Krios control room
TEM microscopes (continued) – Krios operations
Time reserved for data collection of select student projects
15:45 – 17:00 Lecture 8 – SEMC conference room
EMDataResource: Structure Data Archiving, Validation Challenges
Cathy Lawson (Rutgers University)
 [lecture slides][lecture video]
18:00 – 20:00 Reception
Group dinner
 [directions]

Day 5: Friday, March 6, 2020

09:00 – 17:00 Registration/Information desk – SEMC conference room
room available all day except for practicals
09:45 – 10:45 Lecture 9 – NYSBC conference room
Fitting Atomic Models
Oli Clarke (Columbia University)
 [lecture slides][lecture video]
10:45 – 11:45 Lecture 10 – NYSBC conference room
Coordinate Refinement and Validation
Gira Bhabha & Damian Ekiert (New York University)
 [lecture slides][lecture video]
11:45 – 12:00 Practical 5 setup – SEMC conference room
Making biological conclusions from cryoEM reconstructions.
Oli Clarke
 [pre-practical instructions]
12:00 Lunch (pizza lunch) – SEMC conference room
working lunch through practical
12:00 – 15:45 Practical 5 – SEMC conference room
Coot and model building
Oli Clarke & SEMC Staff
 [practical slides]
13:45 – 15:45
by appointment
Practical 3D – Krios control room
TEM microscopes (continued) – Krios operations
Time reserved for data collection of select student projects
SEMC/NCCAT Staff
15:45 – 16:15 Closing and farewell