March 2-6, 2020.
At no cost.
Room and board NOT included.
Note, there is limited on-site availbility. Early registration deadline is November 30, 2019. After this cut-off applicants will automatically be placed on the waitlist.
Please do not book travel and housing until you are accepted into the short course. If you are not accepted into the short course, then you have the option of remotely joining the videocast of the morning lectures.
Questions? Email nccatinfo [at] nysbc.org.
Single Particle Short Course
March 2-6, 2020
NCCAT will be offering a 1 week workshop focused on the theory and practice of single-particle analysis. The mornings will have lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Day 1: Monday, March 2, 2020
- Roundtable : Welcome
- Lecture1 : Keynote – Intro and overview of SPA – Joachim Frank (Columbia University)
- Lecture2 : cryoVR sample preparation – Brenda Gonzalez & Jiahui Dong (Purdue University)
- Practical1 : Support films and grids
- Practical2 : Plunge freezers and Chameleon
Day 2: Tuesday, March 3, 2020
- Lecture3 : Microscopes – SEMC staff
- Roundtable : Facility building
- Practical3 : TEM microscopes
Day 3: Wednesday, March 4, 2020
- Lecture4 : Algorithms and foundational math – Fred Sigworth (Yale University)
- Lecture5: Data Analysis and reconstruction workflow – Amedee des Georges (ASRC/CUNY)
- Roundtable : Current algorithms to deal with SPA
- Practical4: SPA RELION/cryoSPARC (in the cloud using AWS)
Day 4: Thursday, March 5, 2020
- Lecture6 : Interpretation and Limitations – Rich Hite (Memorial Sloan Kettering Cancer Center)
- Lecture7 : Validation Methods – Tom Walz (Rockefeller University)
- Roundtable : EM challenges and new frontiers
- Practical5: SPA RELION/cryoSPARC (in the cloud cont)
- Lecture8 : EMDataResource: Structure Data Archiving, Validation Challenges – Cathy Lawson (Rutgers University)
- Practical6 : Validation methods – 3DFSC
- Group dinner
Day 5: Friday, March 6, 2020
- Lecture9 : Moderate resolution interpretation – Gira Bhabha & Damien Ekiert (New York University)
- Lecture10 : Fitting Atomic Models – Oli Clarke (Columbia Univerisity)
- Roundtable : Making biological conclusions from cryoEM reconstructions.
- Practical7 : Coot and model building
- Roundtable : Farewell