Chameleon2020-05-02T21:50:51+00:00

Chameleon

Merit Badge 8

Category: Sample preparation
Sub-category: Cryogenic work

Plunge freezing and instrument certification for SPT Labtech Chameleon.

Theoretical background

Knowledge of the instrument

Safety

Specific safety SOPs for this merit badge.

• NCCAT SOP for work with ethane for plunge freezing
For general safety information see the SOPs in the right bar.

Relevant SDS (Safety Data Sheets)

• Airgas-SDS-LN2-001188
• Airgas-SDS-Ethane-001024

Vendor product information

Sample preparation equipment for electron microscopy – Next generation sample preparation for cryo-EM based on Spotiton

• SPT Labtech Chameleon product page

Overview of the use of the instrument

• SPT Chameleon demo

Theoretical knowledge

Overview of vitrification for high resolution imaging

• Spotiton: New features and applications
• Optimizing “self-wicking” nanowire grids

Center specific guidelines

• N/A

Knowledge test

A pass/fail test

The knowledge assessment test is a shared test between centers and may be updated regularly. If a user has earned the merit badge and policies have been updated, then an updated information sheet would have to be reviewed ahead of booking an instrumentation session.

Demonstration

Center staff training

The instrument demonstration should be scheduled with center staff through the NCCAT User Office or your appointed user coordinator.

Practice

Supervised and unsupervised practice by the user

Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.

Practical test

Two (2) separate center staff observing your proficiency at separate times

Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.

Additional information

Recertification period

  • Sample preparation merit badges for cryogenic work are valid for ~6mo.
  • Recertification (to maintain active status) would entail demonstrating proper use and care in front of a center staff member.