Tomography Short Course
March 31-April 4, 2025
NCITU & NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. Cryo-electron tomography (cryo-ET) is still emerging as a technique for directly visualizing macromolecular associations and organization in native cells. This course will step through each stage of the cryo-ET workflow (specimen preparation, data acquisition, computational processing, and national infrastructure) and present methodologies that participating trainees could implement at their home institutions. The course curriculum will cover cutting-edge methods in cryo-ET and help attendees accelerate their research with exposure to these methods. The mornings involve lectures and stimulating round table discussions. The afternoons offer hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day. Users are welcome to bring their own samples and/or data.
Short-course Agenda
Monday, March 31, 2025 |
|||
| Session | Type | Lecturer | Topic |
| Morning | Lecture 1 | TBD | Intro and overview of tomography |
| Morning | Lecture 2 | TBD | Cryo sample preparation and FIB-milling for cryoET investigations |
| Morning | Roundtable 1 | Students | Flashtalks |
| Lunch | all attendees | Lunch | |
| Afternoon | Practical 1 | National center staff | Sample preparation practicals and demos |
Tuesday, April 1, 2025 |
|||
| Session | Type | Lecturer | Topic |
| Morning | Lecture 3 | TBD | CryoET data acquisition strategies and software |
| Morning | Roundtable 2 | National center staff | Choosing the right in situ target and model system Choosing the right hardware and scope setup |
| Lunch | all attendees | Lunch | |
| Afternoon | Practical 2 | National center staff | cryoET data collection and software |
Wednesday, April 2, 2025 |
|||
| Session | Type | Lecturer | Topic |
| Morning | Lecture 4 | TBD | Tomography data processing workflows |
| Morning | Roundtable 3 | National center staff | Data processing: segmentation and sub-volume averaging pipelines |
| Morning | Practical 3 | TBD | Sub-Tomogram Alignment and Dynamo |
| Lunch | all attendees | ||
| Afternoon | Practical 4 | TBD | AreTomo + nextPYP |
| Afternoon | Practical (cont) | National center staff | Additional time for data collection and sample preparation |
Thursday, April 3, 2025 |
|||
| Session | Type | Lecturer | Topic |
| Morning | Lecture 5 | TBD | EMAN2 tools, sub-tomogram averaging and annotation |
| Morning | Lecture 6 | Jack Turner (EMDB & EMPIAR) | archiving of cryo-ET data and results in EMDB and EMPIAR |
| Morning | Practical 5 | TBD | EMAN2 tutorial |
| Lunch | all attendees | Lunch | |
| Afternoon | Practical 5 (cont) | TBD | Segmentation and Dragonfly tutorial |
Friday, April 4, 2025 |
|||
| Session | Type | Lecturer | Topic |
| Morning | Roundtable 4 | National center staff | EM challenges and new frontiers What’s next – applying to NIH common fund cryoEM and cryoET centers |
| Morning | Practical 6 | National center staff | Choose your adventure: deeper dive into processing, waffle milling, cryoCLEM & PRIMO |
| Lunch | all attendees | Lunch and farewell | |
| Afternoon | bonus time | all attendees | additional time for cryoET data collection |
Yes. This will be an in-person on-site workshop.
A copy of the NYSBC’s COVID-19 policies may be found here.
Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.