TFS Autoloaders
Merit Badge 9
Category: Sample preparation
Sub-category: Cryogenic work
Autoloader grid handling including clipping, loading, recovery, cassette care and nanocab care.
Theoretical background
Knowledge of the workflow
Safety
Specific safety SOPs for this merit badge.
• NCCAT SOP for work with small amounts of LN2
For general safety information see the SOPs in the right bar.
Relevant SDS (Safety Data Sheets)
Vendor product information
Autoloader compatible TEMs
Theoretical knowledge
Curriculumn development partner modules
Overall workflow
• Getting Started in CryoEM – Unit 2: Sample Preparation [Assembling autogrids, Loading autogrids into cassettes, Docking and undocking capsules modules]
Step by step instructions by Jonathan Ipsaro
• Cryo-EM Sample Preparation: Part II – Grid Clipping
Center specific guidelines
• N/A
Knowledge test
A pass/fail test
The knowledge assessment test is a shared test between centers and may be updated regularly. If a user has earned the merit badge and policies have been updated, then an updated information sheet would have to be reviewed ahead of booking an instrumentation session.
Demonstration
Center staff training
The instrument demonstration should be scheduled with center staff through the NCCAT User Office or your appointed user coordinator.
Practice
Supervised and unsupervised practice by the user
Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.
Practical test
Two (2) separate center staff observing your proficiency at separate times
Center staff will assist in arranging dedicated time slots for access to the necessary equipment. Registered users will be issued scheduler accounts through the NCCAT User Office.
Additional information
Recertification period
- Sample preparation merit badges for cryogenic work are valid for ~6mo.
- Recertification (to maintain active status) would entail demonstrating proper use and care in front of a center staff member.
General NYSBC/NCCAT safety SOPs
Furthering your knowledge
General background on safety