CryoEM Current Practices Webinar 05/27/2021
The next installment of the monthly methods-focused webinar series hosted by the National Center for CryoEM Access and Training (NCCAT), along with the Pacific Northwest Center for CryoEM (PNCC), and the Stanford-SLAC CryoEM Center (S2C2) will be held on Thursday May 27, 2021 at 12 PM eastern / 9 AM pacific time Nicolas Coudray, Senior Scientist, Bhabha/Ekiert Lab New York Univerity School of Medicine will present: Behind the scenes: CryoEM of E. coli lipid transporters Talk Abstract: The outer membrane (OM) of Gram-negative bacteria is an asymmetric bilayer composed of LPS in the outer leaflet and phospholipids in the inner leaflet. It constitutes a robust barrier for the cell, often making bacteria resistant to antibiotics that are unable to cross this [...]
NCCAT roundtables – 2021 edition
Come hang out in our virtual conference room and chat about CryoEM. Starting at the end of April, every other Tuesday afternoon at 3:30 PM eastern time we pick up a new topic. Over the course of the spring and summer we will span many aspects of a typical CryoEM workflow. You don't need to come all the way to New York City to pull up a seat with our local experts, ask your own questions and learn something new. You do need to register to get a zoom link, and bring your own snacks, if needed. All are welcome to attend. Dates and topics Register here
CryoEM Current Practices Webinar 04/29/2021
The next installment of the monthly methods-focused webinar series hosted by the National Center for CryoEM Access and Training (NCCAT), along with the Pacific Northwest Center for CryoEM (PNCC), and the Stanford-SLAC CryoEM Center (S2C2) will be held on Thursday April 29, 2021 at 12 PM eastern / 9 AM pacific time Dr. Rahul Jaiswal, Instructor, Virginia Commonwealth University School of Medicine will talk about his work on Cryo-EM studies of a malleable oligomeric SF3 helicase Talk Abstract: The adeno-associated virus (AAV) non-structural Rep proteins catalyze all the DNA transactions required for virus viability including, DNA replication, transcription regulation, genome packaging, and during a latent phase, site-specific integration. Rep proteins contain two multifunctional domains: An Origin Binding Domain (OBD) and a SF3 [...]
CryoEM Current Practices Webinar 03/25/2021
The next installment of the monthly methods-focused webinar series hosted by the National Center for CryoEM Access and Training (NCCAT), along with the Pacific Northwest Center for CryoEM (PNCC), and the Stanford-SLAC CryoEM Center (S2C2) will be held on Thursday March 25, 2021 at 12 PM eastern / 9 AM pacific time Dr. Richard H. Ebright, Board of Governors Professor of Chemistry and Chemical Biology, Rutgers University will talk about his work on the Structural basis of transcription-translation coupling Talk Abstract: In bacteria, transcription and translation are coupled processes, in which movement of RNA polymerase (RNAP) synthesizing mRNA is coordinated with movement of the first ribosome translating mRNA. Coupling is modulated by the transcription factors NusG--which is thought to bridge RNAP and [...]
CryoEM Current Practices Webinar 02/25/2021
The next installment of the monthly methods-focused webinar series hosted by the National Center for CryoEM Access and Training (NCCAT), along with the Pacific Northwest Center for CryoEM (PNCC), and the Stanford-SLAC CryoEM Center (S2C2) will be held on Thursday February 25, 2021 at 12 PM eastern / 9 AM pacific time Bobby Hollingsworth, Graduate Student, Hao Wu Lab, Harvard Medical School will talk about his work on Workflow tricks for determining cryo-EM structures of filaments, small proteins, and protein complexes Talk Abstract: Moving through a cryo-EM project can be daunting, and issues might arise at every step of the process from sample preparation to preferential orientation on cryo-EM grids and molecular modelling. Here, I will briefly discuss our recent structural advances of [...]