Bobby Hollingsworth, Graduate Student, Hao Wu Lab, Harvard Medical School will talk about his work on Workflow tricks for determining cryo-EM structures of filaments, small proteins, and protein complexes
Talk Abstract: Moving through a cryo-EM project can be daunting, and issues might arise at every step of the process from sample preparation to preferential orientation on cryo-EM grids and molecular modelling. Here, I will briefly discuss our recent structural advances of CARD8 and NLRP1 inflammasomes and then emphasize the technical challenges we encountered and the strategies we employed to overcome them. While by no means exhaustive, these tricks are broadly applicable to a range of cryo-EM workflows. Finally, I will touch on strategies for enhancing materials and data sharing to reduce research redundancy. A copy of this presentation will be made available on our Open Science Framework (OSF) repository: https://osf.io/x7dv8/.