The next installment of the monthly methods-focused webinar series hosted by the National Center for CryoEM Access and Training (NCCAT), along with the Pacific Northwest Center for CryoEM (PNCC), and the Stanford-SLAC CryoEM Center (S2C2) will be held on Thursday February 25, 2021 at 12 PM eastern / 9 AM pacific time

Bobby Hollingsworth, Graduate Student, Hao Wu Lab, Harvard Medical School will talk about his work on Workflow tricks for determining cryo-EM structures of filaments, small proteins, and protein complexes

Talk Abstract: Moving through a cryo-EM project can be daunting, and issues might arise at every step of the process from sample preparation to preferential orientation on cryo-EM grids and molecular modelling. Here, I will briefly discuss our recent structural advances of CARD8 and NLRP1 inflammasomes and then emphasize the technical challenges we encountered and the strategies we employed to overcome them. While by no means exhaustive, these tricks are broadly applicable to a range of cryo-EM workflows. Finally, I will touch on strategies for enhancing materials and data sharing to reduce research redundancy. A copy of this presentation will be made available on our Open Science Framework (OSF) repository:

All are welcome to attend. Registration is at no-cost, but sign-up is required: