• 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • April 12-16, 2027.

  • students/postdocs $375.
    staff/PIs/professionals $450.
    industry $625.

  • Room and board NOT included.

Registration coming soon.

For more information email nccatuseroffice [at] nysbc.org

Full Agenda

Daily schedule

coming soon

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Short-course FAQ

FAQ.
see the 2025 shortcourse

Tomography Short Course
April 12-16, 2026

NCITU & NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. Cryo-electron tomography (cryo-ET) is still emerging as a technique for directly visualizing macromolecular associations and organization in native cells. This course will step through each stage of the cryo-ET workflow (specimen preparation, data acquisition, computational processing, and national infrastructure) and present methodologies that participating trainees could implement at their home institutions. The course curriculum will cover cutting-edge methods in cryo-ET and help attendees accelerate their research with exposure to these methods. The mornings involve lectures and stimulating round table discussions. The afternoons offer hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day. The 2023 offering resulted in a review on cryo-ET workflows (https://doi.org/10.3389/fmolb.2023.1296941). The 2025 YouTube playlist may be found here.

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Short-course Agenda

Monday, April 12, 2027
Session Type Lecturer Topic
Morning Keynote TBD Intro and overview of tomography
Morning Lecture 1 TBD Intro to technologies for cryo sample prep
Morning Lecture 2 TBD Applications of FIB-milling for cryoET investigations
Morning Roundtable 1 Students Flashtalks
Lunch all attendees Lunch
Afternoon Lecture 3 TBD Sample preparation workflows
Afternoon Practical 1 National Center staff Sample preparation practicals and demos

Tuesday, April 13, 2027
Session Type Lecturer Topic
Morning Lecture 4 TBD CryoET data acquisition strategies and software
Morning Lecture 5 & Practical 2 TBD Tomography processing pipeline
Lunch all attendees Lunch
Afternoon Lecture 6 & Practical 3 TBD Cryo-ET data processing tutorial

Wednesday, April 14, 2027
Session Type Lecturer Topic
Morning Roundtable 2 TBD Cryo-ET data processing tutorial (cont)
Morning Lecture 7 Jake Johnston (Columbia University) Data processing: segmentation and sub-volume averaging pipelines
Morning Lecture 8 TBD TBD
Lunch all attendees
Afternoon Practical 4 TBD TBD

Thursday, April 15, 2027
Session Type Lecturer Topic
Morning Lecture 9 TBD CryoET annotation and analysis & AMIRA demo
Morning Roundtable 3  National Center staff EM challenges and new frontiers
Morning Lecture 10 & Practical 5 TBD TBD
Lunch all attendees Lunch
Afternoon Practical 5 (cont) TBD CryoET annotation practical

Friday, April 16, 2027
Session Type Lecturer Topic
Morning Roundtable 4 National Center staff What’s next – applying to NIH common fund cryoEM and cryoET centers
Morning Practical 6 National Center staff Choose your adventure: deeper dive into waffle milling, cryoCLEM & data collection
Wrap up all attendees farewell
Afternoon bonus time all attendees additional time for cryoET data collection

This workshop is limited up to 12 attendees and accepted applicants will receive instructions for payment of the course fee.

Email the NCCAT User Office for more information.

We will reach out to participants early January and waitlist participants by the end of January. For further inquires please email the NCCAT User Office.

Yes. This will be an in-person on-site workshop.

See the 2023 offering of this workshop here.

Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.