1 week.
Morning lectures.
Afternoon practicals.
April 12-16, 2027.
students $375.
postdocs $425.
staff/PIs/professionals $600.
industry $875.Room and board NOT included.
- Early application deadline: January 11, 2027.
- Travel award application deadline: February 12, 2027.
- Late application/registration deadline: March 12, 2027.
- Acceptances will begin mid-January on a rolling basis.
- Waitlisted applicants will be informed starting in late February.
- For more information, email nccatuseroffice [at] nysbc.org
Tomography Short Course
April 12-16, 2027
NCITU & NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. Cryo-electron tomography (cryo-ET) is still emerging as a technique for directly visualizing macromolecular associations and organization in native cells. This course will step through each stage of the cryo-ET workflow (specimen preparation, data acquisition, computational processing, and national infrastructure) and present methodologies that participating trainees could implement at their home institutions. The course curriculum will cover cutting-edge methods in cryo-ET and help attendees accelerate their research with exposure to these methods. The mornings involve lectures and stimulating round table discussions. The afternoons offer hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day. The 2023 offering resulted in a review on cryo-ET workflows (https://doi.org/10.3389/fmolb.2023.1296941). The 2025 YouTube playlist may be found here.
sponsored by
Short-course Agenda
Monday, April 12, 2027 |
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| Session | Type | Lecturer | Topic |
| Morning | Keynote | Wei Dai (Rutgers University) | Intro and overview of tomography |
| Morning | Lecture 1 | Greg Alushin & Keith Hamilton (Rockefeller University) | Intro to technologies for cryo sample prep |
| Morning | Lecture 2 | Alex de Marco (NYSBC) | Applications of FIB-milling for cryoET investigations |
| Morning | Roundtable 1 | Students | Flashtalks |
| Lunch | all attendees | Lunch | |
| Afternoon | Tour | Students | Tour of NYSBC |
| Afternoon | Practical 1 | National Center staff | cryoCLEM practicals & sample preparation practicals/demos |
| Evening | Reception | all attendees | Reception at NYSBC |
Tuesday, April 13, 2027 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 3 | Geoff Perumal (Thermo Fisher Scientific) | cryoET workflows and instrumentation |
| Morning | Lecture 4 | Daija Bobe (NYSBC) | cryoFIB milling strategies |
| Morning | Lecture 5 | Jasmine Nguyen (NYSBC) / Rohit Kannachel (NYSBC) | Lift-out considerations / Automation strategies |
| Morning | Practical 2 | National Center Staff | cryoFIB demos |
| Lunch | all attendees | Lunch | |
| Afternoon | Lecture 6 | Eugene Chua (NYSBC) | Data collection strategies and square aperture |
| Afternoon | Practical 3 | National Center Staff | Krios/Glacios setup and tomography acquisition |
Wednesday, April 14, 2027 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 7 | Alberto Bartesaghi (Duke University) | Tomography data processing with nextPYP |
| Morning | Lecture 8 | Jake Johnston (Columbia University) | Data processing: segmentation and sub-volume averaging pipelines |
| Morning | Practical 4 | Alberto Bartesaghi (Duke University) | NextPYP Practical |
| Lunch | all attendees | ||
| Afternoon | Practical 4 (continued) | Alberto Bartesaghi (Duke University) | NextPYP Practical (continued) |
Thursday, April 15, 2027 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 9 | Arsalan Zolfaghari (Thermo Fisher Scientific) | CryoET annotation and analysis with Amira |
| Morning | Lecture 10 | Will Conway (Flatiron/NYSBC) & Gabriel Meyer-Lee (NYSBC) | TARDIS for filament picking |
| Morning | Lecture 11 & Practical 5 | Willy Wriggers (Old Dominion University) & Salim Sazzed (Georgia Southern) | Tomography tools in Situs and practical prep |
| Lunch | all attendees | Lunch | |
| Afternoon | Practical 5 (continued) | Willy Wriggers (Old Dominion University) & Salim Sazzed (Georgia Southern) | Tomography tools in Situs practical |
| Evening | all attendees | Group dinner | |
Friday, April 16, 2027 |
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| Session | Type | Lecturer | Topic |
| Morning | Practical 6 | Arsalan Zolfaghari (Thermo Fisher Scientific) | During this 3-hour workshop, the instructor will guide you through a workflow for analyzing cryo-EM images. You’ll work hands-on in your own Amira session: processing your data, segmenting features of interest, extracting measurements, and exploring the results using tables, charts, and visualizations projected back into the 3D space of your segmented objects. You will be given access to the latest version of Amira running on AWS. Please bring a laptop; an external mouse is recommended. If you have a challenging dataset and would like the instructor’s input, bring it on a USB drive or upload it to a server. There will be time to discuss your dataset and possible analysis approaches at the end of the workshop. Thermo Fisher provides Amira licenses on AWS-compatible hardware, so space is limited. Please sign up to reserve a spot in this hands-on workshop, tailored to cryo-EM image processing, analysis, and visualization. |
| Morning | Roundtable | National Center staff | What’s next – applying to NIH common fund cryoEM and cryoET centers |
| Wrap up | all attendees | farewell | |
| Afternoon | bonus time | all attendees | additional time Choose your adventure: deeper dive into waffle milling, cryoCLEM & data collection |
Yes. This will be an in-person on-site workshop.
See the 2025 offering of this workshop here.
Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.
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NIH style biosketch (https://www.ncbi.nlm.nih.gov/sciencv/)
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Main application: No more than 2 pages covering a) your current research, b) your career goals, and c) how this course would further your professional development.
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Proof of Status: For trainees, a copy of your current valid student/university ID will suffice. For professionals, include your NIH Current and Pending (Other) Support Common Form.