1 week.
Morning lectures.
Afternoon practicals.
March 16-20, 2026.
students/postdocs $375.
staff/PIs/professionals $450.
industry $625.Room/Accomodations NOT included.
Standard applications will close December 15, 2025. Late applications will be considered up to January 2, 2026.
Accepted applicants will be notified early January 2026.
For more information email nccatuseroffice [at] nysbc.org
Single Particle Short Course
March 16-20, 2026
NCCAT will offer a 1 week workshop focused on the theory and practice of single-particle analysis. The mornings are filled with lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Short-course Agenda
Monday, March 16, 2026 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 1 – Keynote | Joachim Frank (Columbia University) | Intro and overview of SPA [Lecture slides] |
| Morning | Roundtable 1 | students | Flashtalks from students |
| Afternoon | Lecture 2 | SEMC staff | Sample preparation for biological samples [Lecture slides] |
| Afternoon | Practical 1 | SEMC staff | CryoEM sample prep and grid prep stations [Grid prep notes sheet][Test Samples Info Page] |
Tuesday, March 17, 2026 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 3a | SEMC staff | Microscopes and tools of the trade [Lecture slides] |
| Morning | Lecture 3b | SEMC staff | Maintenance of cryoEM facilities [Lecture slides] |
| Morning | Rapid demos 1 | SEMC staff | Cutting-edge/advanced topics in EM |
| Afternoon | Roundtable 2 | SEMC staff | Making use of local facilities and national centers [Slides] |
| Afternoon | Practical 2 | SEMC staff | Efficient grid preparation & screening on a TEM |
Wednesday, March 18, 2026 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 4a | Fred Sigworth (Yale University) | Algorithms and foundational math PartI: derivation/explanation of the CTF, FTs. [Lecture Slides] |
| Morning | Lecture 4b | Fred Sigworth (Yale University) | Algorithms and foundational math PartII: reconstruction, classification, and maximum likelihood. [Lecture Slides] |
| Morning | Lecture 5 | David Jeruzalmi (City University of New York) | Data analysis and reconstruction workflow [Lecture Slides] |
| Lunch | Roundtable 3 | Fred Sigworth & David Jeruzalmi | Current and new algorithms to deal with SPA reconstruction |
| Afternoon | Practical 3 | SEMC staff | Processing using cryoSPARC [Tutorial Document] |
Thursday, March 19, 2026 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 6 | Rich Hite (Memorial Sloan Kettering Cancer Center) | Interpretation and Limitations of SPA [Lecture Slides] |
| Morning | Lecture 7 | Andres Leschziner (Weill Cornell School of Medicine) | Applications of cryoEM in the life sciences [Lecture Slides] |
| Lunch | Roundtable 4 | Rich Hite & Andres Leschziner | EM challenges and new frontiers |
| Afternoon | Lecture 8 | Anthony Fitzpatrick (Columbia University) | New electron microscopy hardware and single-particle approaches for imaging in situ |
| Afternoon | Practical 4 | SEMC staff | Autoloader TEMs and Krios data collection at a national center |
| Dinner | all instructors | Group dinner | |
Friday, March 20, 2026 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 9 | Oli Clarke (Columbia University) | Fitting Atomic Models [Lecture slides] |
| Morning | Lecture 10 | Justin Flatt (RSCB/PDB) | Best Practices for Cryo-EM Deposition and Validation [Lecture slides] |
| Morning | Roundtable 5 | Oli Clarke & Justin Flatt | Making cryoEM information accessible to the community. |
| Afternoon | Practical 5 | Oli Clarke | Coot and model building: [Tutorial PDF] or [Tutorial html] |
Yes. This will be an in-person on-site workshop.
Yes. Parts of the workshop will be recorded. We will post all learning materials on our website.
Accepted participants will be charged for this course. Travel, food and housing costs are not covered. Instructions will follow after acceptance.
students/postdocs: $375.
staff/PIs/professionals: $450.
industry: $625.
If participants cancel or withdraw from the short course:
- One month ahead of the workshop, there will be a refund of up to 66% minus processing fees,
- Two weeks ahead of the workshop, there will be a refund of up to 50% minus processing fees,
- One week ahead of the workshop, there will be no refunds.