Tomography Short course2020-01-29T21:16:13+00:00

 

  • 1 week.

  • Morning lectures.

  • Afternoon practicals.

  • April 13-17, 2020.

  • At no cost.

  • Room and board not included.

Note, the registration for the short-course has closed. Students not accepted into the short course have the option of joining the videocast of the morning lectures. Use the registration link to be added to the email list.

Questions? Email nccatinfo [at] nysbc.org.

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Sponsors

Tomography Shortcourse

April 13-17, 2020

SEMC and NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. The mornings will have lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.

Short-course Agenda ver. 2020.01.27

Day 1: Monday, April 13, 2020

09:00 – 09:30 Registration and Welcome – SEMC conference room
09:50 – 10:00 Opening – NYSBC conference room
10:00 – 11:15 Keynote / Lecture 1 – NYSBC conference room
Intro and overview of ET
Wei Dai (Rutgers University)
11:15 – 11:30 Break
11:30 – 12:15 Roundtable 1 – SEMC conference room
Introductions and course overview
Short course attendees
12:15 – 13:15 Lunch
13:15 – 17:00 Practical 1 – SEMC conference room
ET sample preparation – HPF/Plunge freezers

Practical 2 – SEMC conference room
cryoCLEM-FIB-ET workflow
SEMC/NCCAT Staff

Day 2: Tuesday, April 14, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
09:45 – 11:00 Lecture 2 – NYSBC conference room
FIB-SEM
Bill Rice (New York University)
11:00 – 11:15 Break
11:15 – 12:00 Roundtable 2 – SEMC conference room
Making windows into cells
Bill Rice & SEMC/NCCAT Staff
12:00 – 13:15 Lunch
13:15 – 17:00 Practical 3 – SEMC conference room
Krios cryoET data collection
SEMC/NCCAT Staff

Day 3: Wednesday, April 15, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
09:45 – 11:00 Lecture 3 – NYSBC conference room
Cryo-Applications and sub-tomogram averaging
Alex Noble (NYSBC/SEMC)
11:00 – 11:15 Break
11:15 – 12:00 Roundtable 3 – SEMC conference room
Choosing the right in situ target and model system
Alex Noble & SEMC/NCCAT Staff
12:00 – 13:15 Lunch
13:15 – 17:00

14:45 – 17:00
by appointment

Practical 4 – SEMC conference room
Appion Protomo tools and sub-tomogram averaging
Alex Noble & SEMC/NCCAT Staff

Practical 3B – SEMC conference room
Krios cryoET data collection (continued)
SEMC/NCCAT Staff

Day 4: Thursday, April 16, 2020

09:00 – 09:30 Registration/Information desk – SEMC conference room
10:00 – 12:00 Lecture 4 – NYSBC conference room
Automated reconstruction, subtomogram averaging methods & data interpretation Steven Ludtke (Baylor College of Medicine)
12:00 – 13:15 Lunch
13:15 – 13:30 Group photo
13:30 – 17:00 Practical 5 – SEMC conference room
EMAN2 tools, sub-tomogram averaging and annotation
Muyuan Chen (Baylor College of Medicine)
14:45 – 17:00
by appointment
Practical 3C – Krios control room
Krios cryoET data collection (continued)
Time reserved for data collection of select student projects
SEMC/NCCAT Staff
18:00 – 20:00 Reception
Group dinner

Day 5: Friday, April 17, 2020

09:00 – 15:30 Registration/Information desk – SEMC conference room
room available all day except for classes
10:00 – 10:45 Roundtable 4 – SEMC conference room
The future of in situ cellular structural biology
SEMC/NCCAT staff
10:45 – 11:15 Closing and farewell
 
11:30 – 15:30
by appointment
Practical 3D – Krios control room
Krios cryoET data collection (continued)
Time reserved for data collection of select student projects
SEMC/NCCAT Staff