1 week.
Morning lectures.
Afternoon practicals.
April 13-17, 2020.
At no cost.
Room and board not included.
Note, the registration for the short-course has closed. Students not accepted into the short course have the option of joining the videocast of the morning lectures. Use the registration link to be added to the email list.
Questions? Email nccatinfo [at] nysbc.org.
Sponsors
Tomography Shortcourse
April 13-17, 2020
Due to COVID-19 this workshop will be postponed until further notice. We will keep you informed about updates after we revise our workshop agenda. We are currently discussing alternatives, but have decided against a remote format and want to hold an in person workshop at an appropriate time.
SEMC and NCCAT will be offering a 1 week workshop focused on the theory and practice of tomographic methods. The mornings will have lectures and stimulating round table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Short-course Agenda ver. 2020.01.27
Day 1: Monday, April 13, 2020
| 09:00 – 09:30 | Registration and Welcome – SEMC conference room |
| 09:50 – 10:00 | Opening – NYSBC conference room |
| 10:00 – 11:15 | Keynote / Lecture 1 – NYSBC conference room Intro and overview of ET Wei Dai (Rutgers University) |
| 11:15 – 11:30 | Break |
| 11:30 – 12:15 | Roundtable 1 – SEMC conference room Introductions and course overview Short course attendees |
| 12:15 – 13:15 | Lunch |
| 13:15 – 17:00 | Practical 1 – SEMC conference room ET sample preparation – HPF/Plunge freezersPractical 2 – SEMC conference room cryoCLEM-FIB-ET workflow SEMC/NCCAT Staff |
Day 2: Tuesday, April 14, 2020
| 09:00 – 09:30 | Registration/Information desk – SEMC conference room |
| 09:45 – 11:00 | Lecture 2 – NYSBC conference room FIB-SEM Bill Rice (New York University) |
| 11:00 – 11:15 | Break |
| 11:15 – 12:00 | Roundtable 2 – SEMC conference room Making windows into cells Bill Rice & SEMC/NCCAT Staff |
| 12:00 – 13:15 | Lunch |
| 13:15 – 17:00 | Practical 3 – SEMC conference room Krios cryoET data collection SEMC/NCCAT Staff |
Day 3: Wednesday, April 15, 2020
| 09:00 – 09:30 | Registration/Information desk – SEMC conference room |
| 09:45 – 11:00 | Lecture 3 – NYSBC conference room Cryo-Applications and sub-tomogram averaging Alex Noble (NYSBC/SEMC) |
| 11:00 – 11:15 | Break |
| 11:15 – 12:00 | Roundtable 3 – SEMC conference room Choosing the right in situ target and model system Alex Noble & SEMC/NCCAT Staff |
| 12:00 – 13:15 | Lunch |
| 13:15 – 17:00
14:45 – 17:00 |
Practical 4 – SEMC conference room Appion Protomo tools and sub-tomogram averaging Alex Noble & SEMC/NCCAT StaffPractical 3B – SEMC conference room Krios cryoET data collection (continued) SEMC/NCCAT Staff |
Day 4: Thursday, April 16, 2020
| 09:00 – 09:30 | Registration/Information desk – SEMC conference room |
| 10:00 – 12:00 | Lecture 4 – NYSBC conference room Automated reconstruction, subtomogram averaging methods & data interpretation Steven Ludtke (Baylor College of Medicine) |
| 12:00 – 13:15 | Lunch |
| 13:15 – 13:30 | Group photo |
| 13:30 – 17:00 | Practical 5 – SEMC conference room EMAN2 tools, sub-tomogram averaging and annotation Muyuan Chen (Baylor College of Medicine) |
| 14:45 – 17:00 by appointment |
Practical 3C – Krios control room Krios cryoET data collection (continued) Time reserved for data collection of select student projects SEMC/NCCAT Staff |
| 18:00 – 20:00 | Reception Group dinner |
Day 5: Friday, April 17, 2020
| 09:00 – 15:30 | Registration/Information desk – SEMC conference room room available all day except for classes |
| 10:00 – 10:45 | Roundtable 4 – SEMC conference room The future of in situ cellular structural biology SEMC/NCCAT staff |
| 10:45 – 11:15 | Closing and farewell |
| 11:30 – 15:30 by appointment |
Practical 3D – Krios control room Krios cryoET data collection (continued) Time reserved for data collection of select student projects SEMC/NCCAT Staff |