Single Particle Short Course
March 11-15, 2024
NCCAT is offering a 1-week workshop focused on the theory and practice of single-particle analysis. The mornings are filled with lectures and stimulating round-table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Monday, March 11, 2024
|Morning||Lecture 1 – Keynote||Joachim Frank (Columbia University)||Intro and overview of SPA|
|Morning||Roundtable 1||students||Flashtalks from students|
|Afternoon||Lecture 2||Victor Chen & Wen Jian (Purdue University)||cryoVR for sample preparation|
|Afternoon||Practical 1||SEMC staff||cryoEM sample prep and grid prep stations|
Tuesday, March 12, 2024
|Morning||Lecture 3||SEMC staff||Microscopes and tools of the trade|
|Afternoon||Roundtable 2||SEMC staff||Making use of local facilities and national centers|
|Afternoon||Practical 2||SEMC staff||Plunge freezing & TEM stations|
Wednesday, March 13, 2024
|Morning||Lecture 4a||Fred Sigworth (Yale University)||Algorithms and foundational math PartI:
derivation/explanation of the CTF, FTs.
|Morning||Lecture 4b||Fred Sigworth (Yale University)||Algorithms and foundational math PartII:
reconstruction, classification, and maximum likelihood.
|Morning||Lecture 5||Amedee des Georges & Reza Khayat (ASRC/CUNY)||Data Analysis and reconstruction workflow|
|Lunch||Roundtable 3||Fred Sigworth & Amedee des Georges||Current and new algorithms to deal with SPA reconstruction|
|Afternoon||Practical 3||SEMC staff||Processing using cryoSPARC|
Thursday, March 14, 2024
|Morning||Lecture 6||Rich Hite (Memorial Sloan Kettering Cancer Center)||Interpretation and Limitations of SPA|
|Morning||Lecture 7||Tom Walz (Rockefeller University)||SPA today and tomorrow [not Validation Methods]|
|Lunch||Roundtable 4||Rich Hite & Tom Walz||EM challenges and new frontiers|
|Afternoon||Practical 4||SEMC staff||Autoloader TEMs and Krios data collection at a national center|
|Afternoon||Lecture 8||Jack Turner||Deposition and Structure Data Archiving|
|Dinner||all instructors||Group dinner|
Friday, March 15, 2024
|Morning||Lecture 9||Oli Clarke (Columbia University)||Fitting Atomic Models|
|Morning||Lecture 10||Gira Bhabha & Damian Ekiert (New York University)||Coordinate Refinement and Validation|
|Morning||Roundtable 5||Oli Clarke, Gira Bhabha and Damian Ekiert||Making biological conclusions from cryoEM reconstructions.|
|Afternoon||Practical 5||Oli Clarke||Coot and model building|
Yes. This will be an in-person on-site workshop.
A copy of the NYSBC’s COVID-19 policies may be found here.
Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.