Single Particle Short Course
March 11-15, 2024
NCCAT is offering a 1-week workshop focused on the theory and practice of single-particle analysis. The mornings are filled with lectures and stimulating round-table discussions. The afternoons provide hands-on practicals to reinforce fundamental concepts and topics covered earlier in the day.
Short-course Agenda
Monday, March 11, 2024 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 1 – Keynote | Joachim Frank (Columbia University) | Intro and overview of SPA |
| Morning | Roundtable 1 | students | Flashtalks from students |
| Afternoon | Lecture 2 | Victor Chen & Wen Jian (Purdue University) | cryoVR for sample preparation |
| Afternoon | Practical 1 | SEMC staff | cryoEM sample prep and grid prep stations |
Tuesday, March 12, 2024 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 3 | SEMC staff | Microscopes and tools of the trade |
| Afternoon | Roundtable 2 | SEMC staff | Making use of local facilities and national centers |
| Afternoon | Practical 2 | SEMC staff | Plunge freezing & TEM stations |
Wednesday, March 13, 2024 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 4a | Fred Sigworth (Yale University) | Algorithms and foundational math PartI: derivation/explanation of the CTF, FTs. |
| Morning | Lecture 4b | Fred Sigworth (Yale University) | Algorithms and foundational math PartII: reconstruction, classification, and maximum likelihood. |
| Morning | Lecture 5 | Amedee des Georges & Reza Khayat (ASRC/CUNY) | Data Analysis and reconstruction workflow |
| Lunch | Roundtable 3 | Fred Sigworth & Amedee des Georges | Current and new algorithms to deal with SPA reconstruction |
| Afternoon | Practical 3 | SEMC staff | Processing using cryoSPARC |
Thursday, March 14, 2024 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 6 | Rich Hite (Memorial Sloan Kettering Cancer Center) | Interpretation and Limitations of SPA |
| Morning | Lecture 7 | Tom Walz (Rockefeller University) | SPA today and tomorrow [not Validation Methods] |
| Lunch | Roundtable 4 | Rich Hite & Tom Walz | EM challenges and new frontiers |
| Afternoon | Practical 4 | SEMC staff | Autoloader TEMs and Krios data collection at a national center |
| Afternoon | Lecture 8 | Jack Turner | Deposition and Structure Data Archiving |
| Dinner | all instructors | Group dinner | |
Friday, March 15, 2024 |
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| Session | Type | Lecturer | Topic |
| Morning | Lecture 9 | Oli Clarke (Columbia University) | Fitting Atomic Models |
| Morning | Lecture 10 | Gira Bhabha & Damian Ekiert (New York University) | Coordinate Refinement and Validation |
| Morning | Roundtable 5 | Oli Clarke, Gira Bhabha and Damian Ekiert | Making biological conclusions from cryoEM reconstructions. |
| Afternoon | Practical 5 | Oli Clarke | Coot and model building |
Yes. This will be an in-person on-site workshop.
A copy of the NYSBC’s COVID-19 policies may be found here.
Yes. All parts of the workshop will be recorded. We will post all learning materials on our website.